OLR1 / LOX1 recombinant protein

Recombinant Human OLR1 / LOX1 Protein (His tag)

Cat. Num. AAA2546168

• Gene Names: OLR1; LOX1; LOXIN; SLOX1; CLEC8A; SCARE1
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: OLR1 / LOX1; Recombinant Human OLR1 / LOX1 Protein (His tag); OLR1; CLEC8A; LOX1; LOXIN; SCARE1; SLOX1; OLR1 / LOX1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 181

• Application Notes: The recombinant human OLR1 consists of 229 amino acids and has a calculated molecular mass of 26.5 kDa. It exists as a disulfide-linked homodimer. As a result of glycosylation, the apparent molecular mass of rhOLR1 is approximately 30-35 kDa in SDS-PAGE under reducing conditions, and 60-70 kDa in non-reduced SDS-PAGE.
• Predicted N Terminal: His
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for OLR1 / LOX1 recombinant protein

Background: Oxidized low-density lipoprotein receptor 1 (Ox-LDL receptor 1 or OLR1), also known as lectin-type oxidized LDL receptor 1 (LOX1), is a receptor protein that belongs to the C-type lectin superfamily. LOX1 is a multi-ligand receptor originally identified as the endothelial oxidized LDL receptor. OLR1 / LOX1 was isolated from an aortic endothelial cell, and recently it has been discovered in macrophages and vascular smooth muscle cells in artery vessels. The expression of LOX1 is inducted by inflammatory stimuli and oxidative stimuli. This protein binds, internalizes and degrades oxidized low-density lipoprotein. LOX1 may play an important role in the progression of vulnerable carotid plaque and might regulate vulnerable plaque formation in cooperation with MMPs and TIMP-2. In clinical, LOX1 is thought to be involved in the development of atherosclerotic lesions. 

Description: A DNA sequence encoding the human OLR1 (P78380) extracellular domain (Ser 61-Gln 273) was expressed, with a polyhistidine tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 290654342
• NCBI GeneID: 4973
• NCBI Accession #: NP_001166103.1
• NCBI GenBank Nucleotide #: NM_001172632.1
• UniProt Accession #: P78380; Q2PP00; Q7Z484; A8K7V9; B4DI48; G3V1I4
• Molecular Weight: 21,425 Da
• NCBI Official Full Name: oxidized low-density lipoprotein receptor 1 isoform 2
• NCBI Official Synonym Full Names: oxidized low density lipoprotein receptor 1
• NCBI Official Symbol: OLR1
• NCBI Official Synonym Symbols: LOX1; LOXIN; SLOX1; CLEC8A; SCARE1
• NCBI Protein Information: oxidized low-density lipoprotein receptor 1
• UniProt Protein Name: Oxidized low-density lipoprotein receptor 1
• UniProt Gene Name: OLR1
• UniProt Synonym Gene Names: CLEC8A; LOX1; Ox-LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1; hLOX-1

NCBI Description

This gene encodes a low density lipoprotein receptor that belongs to the C-type lectin superfamily. This gene is regulated through the cyclic AMP signaling pathway. The encoded protein binds, internalizes and degrades oxidized low-density lipoprotein. This protein may be involved in the regulation of Fas-induced apoptosis. This protein may play a role as a scavenger receptor. Mutations of this gene have been associated with atherosclerosis, risk of myocardial infarction, and may modify the risk of Alzheimer's disease. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]

Uniprot Description

OLR1: Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro- oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro- atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram- positive bacteria. Independent association genetic studies have implicated OLR1 gene variants in myocardial infarction susceptibility. OLR1 may be involved in Alzheimer disease (AD). Involvement in AD is however unclear: according to some authors (PubMed:12354387, PubMed:12810610 and PubMed:15976314), variations in OLR1 modify the risk of AD, while according to other (PubMed:15000751 and PubMed:15060104) they do not. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 12p13.2

Cellular Component: integral to plasma membrane; intracellular membrane-bound organelle; membrane; nucleoplasm; plasma membrane; receptor complex

Molecular Function: protein binding

Biological Process: blood circulation; leukocyte migration; proteolysis

Disease: Myocardial Infarction, Susceptibility To