CSF2RA / GM-CSFR / CD116 recombinant protein

Recombinant Human CSF2RA / GM-CSFR / CD116 Protein (His tag)

Cat. Num. AAA2546125

• Gene Names: CSF2RA; GMR; CD116; CSF2R; SMDP4; CDw116; CSF2RX; CSF2RY; GMCSFR; CSF2RAX; CSF2RAY; GM-CSF-R-alpha
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: CSF2RA / GM-CSFR / CD116; Recombinant Human CSF2RA / GM-CSFR / CD116 Protein (His tag); CD116; Csfgmra; GM-CSFR; GM-CSF-Ra; Csf2ra; CSF2RA / GM-CSFR / CD116 recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 400

• Application Notes: The recombinant human GM-CSFRalpha consists of 309 amino acids and has a predicted molecular mass of 36 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh GM-CSFRalpha is approximately 60-65 kDa due to glycosylation.
• Predicted N Terminal: Glu 23
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CSF2RA / GM-CSFR / CD116 recombinant protein

Background: CD116/GM-CSFR has been preferentially associated with M4, M5 subtype of AML but is not specific. The cluster of differentiation (cluster of designation) (often abbreviated as CD) is a protocol used for the identification and investigation of cell surface molecules present on white blood cells initially but found in almost any kind of cell of the body, providing targets for immunophenotyping of cells. Physiologically, CD molecules can act in numerous ways, often acting as receptors or ligands (the molecule that activates a receptor) important to the cell. A signal cascade is usually initiated, altering the behavior of the cell (see cell signaling). Some CD proteins do not play a role in cell signaling, but have other functions, such as cell adhesion. CD116/GM-CSFR is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. CD116/GM-CSFR is found in the pseudoautosomal region (PAR) of the X and Y chromosomes.

Description: A DNA sequence encoding the extracellular domain (Met 1-Gly 320) of human GM-CSFRalpha (P15509-1) was expressed with the C-terminal fused polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 238908515
• NCBI GeneID: 1438
• NCBI Accession #: NP_001155001.1
• NCBI GenBank Nucleotide #: NM_001161529.1
• UniProt Accession #: P15509; O00207; Q14429; Q14430; Q14431; Q16564; A7J003; A8KAM1; B4DW68; J3JS76; J3JS77
• Molecular Weight: 31,101 Da
• NCBI Official Full Name: granulocyte-macrophage colony-stimulating factor receptor subunit alpha isoform a
• NCBI Official Synonym Full Names: colony stimulating factor 2 receptor alpha subunit
• NCBI Official Symbol: CSF2RA
• NCBI Official Synonym Symbols: GMR; CD116; CSF2R; SMDP4; CDw116; CSF2RX; CSF2RY; GMCSFR; CSF2RAX; CSF2RAY; GM-CSF-R-alpha
• NCBI Protein Information: granulocyte-macrophage colony-stimulating factor receptor subunit alpha
• UniProt Protein Name: Granulocyte-macrophage colony-stimulating factor receptor subunit alpha
• UniProt Gene Name: CSF2RA
• UniProt Synonym Gene Names: CSF2R; CSF2RY; GM-CSF-R-alpha; GMCSFR-alpha; GMR-alpha

NCBI Description

The protein encoded by this gene is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. This gene is found in the pseudoautosomal region (PAR) of the X and Y chromosomes. Multiple transcript variants encoding different isoforms have been found for this gene, with some of the isoforms being membrane-bound and others being soluble. [provided by RefSeq, Jul 2008]

Uniprot Description

CSF2RA: Low affinity receptor for granulocyte-macrophage colony- stimulating factor. Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells. Defects in CSF2RA are the cause of pulmonary surfactant metabolism dysfunction type 4 (SMDP4). A rare lung disorder due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress. Belongs to the type I cytokine receptor family. Type 5 subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: Xp22.33 and Yp11.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; receptor activity

Biological Process: cellular protein metabolic process; MAPKKK cascade

Disease: Surfactant Metabolism Dysfunction, Pulmonary, 4