beta-Catenin / CTNNB1 Recombinant Protein | CTNNB1 recombinant protein

Recombinant Human beta-Catenin / CTNNB1 Protein (His & GST tag)

Cat. Num. AAA2545831

• Gene Names: CTNNB1; CTNNB; MRD19; armadillo
• Purity: > 87 % as determined by SDS-PAGE
• Synonyms: beta-Catenin / CTNNB1; Recombinant Human beta-Catenin / CTNNB1 Protein (His & GST tag); OK/SW-cl.35; CTNNB; DKFZp686D02253; FLJ25606; FLJ37923; CTNNB1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 87 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, 2mM GSH, 10% gly, pH 8.0
• Sequence Length: 781

• Application Notes: The recombinant human CTNNB1/GST chimera consists of 1018 amino acids and has a calculated molecular mass of 113 kDa. It migrates as an approximately 116 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CTNNB1 recombinant protein

Background: beta-Catenin, also known as CTNNB1, is a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain, which is specialized for protein-protein binding. It is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. CTNNB1 also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, beta-Catenin binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Defects in beta-Catenin can cause colorectal cancer, pilomatrixoma (PTR), medulloblastoma, and ovarian cancer. CTNNB1 is a key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, beta-Catenin is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. CTNNB1 is involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.

Description: A DNA sequence encoding the human CTNNB1 (P35222-1) (Met 1-Leu 781) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 148233338
• NCBI GeneID: 1499
• NCBI Accession #: NP_001091679.1
• NCBI GenBank Nucleotide #: NM_001098209.1
• UniProt Accession #: P35222; Q8NEW9; Q8NI94; Q9H391; A8K1L7
• Molecular Weight: 9,501 Da
• NCBI Official Full Name: catenin beta-1 isoform 1
• NCBI Official Synonym Full Names: catenin beta 1
• NCBI Official Symbol: CTNNB1
• NCBI Official Synonym Symbols: CTNNB; MRD19; armadillo
• NCBI Protein Information: catenin beta-1
• UniProt Protein Name: Catenin beta-1
• Protein Family: Catenin
• UniProt Gene Name: CTNNB1
• UniProt Synonym Gene Names: CTNNB

NCBI Description

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]

Uniprot Description

CTNNB1: a regulator of cell adhesion and a key downstream effector in the Wnt signaling pathway. Implicated early embryonic development and tumorigenesis. Phosphorylated and destabilized by CK1 and GSK-3beta. Stabilized cytoplasmic beta-catenin is a hallmark of a variety of cancers. Stabilized beta-catenin translocates to the nucleus, where it acts as a transcriptional activator of T-cell factor (TCF)-regulated genes. Interacts with the PDZ domain of TAX1BP3, inhibiting its transcriptional activity. Two alternatively spliced human isoforms have been described.

Protein type: Actin-binding; Cell adhesion; Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 3p22.1

Cellular Component: adherens junction; basolateral plasma membrane; beta-catenin destruction complex; catenin complex; cell cortex; cell junction; cell-cell adherens junction; centrosome; cytoplasm; cytosol; focal adhesion; intercellular junction; lateral plasma membrane; membrane; nucleoplasm; nucleus; perinuclear region of cytoplasm; plasma membrane; protein complex; transcription factor complex

Molecular Function: alpha-catenin binding; cadherin binding; enzyme binding; estrogen receptor binding; kinase binding; nuclear hormone receptor binding; protein binding; protein C-terminus binding; protein phosphatase binding; SMAD binding; transcription coactivator activity; transcription factor binding

Biological Process: cell adhesion; cell-cell adhesion; epithelial to mesenchymal transition; hair cell differentiation; negative regulation of cell proliferation; negative regulation of mitotic cell cycle, embryonic; negative regulation of protein sumoylation; negative regulation of transcription, DNA-dependent; patterning of blood vessels; positive regulation of apoptosis; positive regulation of histone H3-K4 methylation; positive regulation of interferon type I production; positive regulation of muscle cell differentiation; positive regulation of neuroblast proliferation; positive regulation of neuron apoptosis; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; proteasomal ubiquitin-dependent protein catabolic process; regulation of angiogenesis; regulation of centriole-centriole cohesion; regulation of fibroblast proliferation; regulation of neurogenesis; regulation of smooth muscle cell proliferation; response to drug; response to estradiol stimulus; stem cell maintenance; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin; Wnt receptor signaling pathway, calcium modulating pathway

Disease: Colorectal Cancer; Hepatocellular Carcinoma; Mental Retardation, Autosomal Dominant 19; Ovarian Cancer; Pilomatrixoma