ALDH1 / ALDH1A1 / ALDC recombinant protein

Recombinant Human ALDH1 / ALDH1A1 / ALDC Protein (His tag)

Cat. Num. AAA2545795

• Gene Names: ALDH1A1; ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e
• Purity: > 98 % as determined by SDS-PAGE
• Synonyms: ALDH1 / ALDH1A1 / ALDC; Recombinant Human ALDH1 / ALDH1A1 / ALDC Protein (His tag); ALDH1A1; RP11-151D14.2; ALDC; ALDH-E1; ALDH1; ALDH11; MGC2318; PUMB1; RALDH1; ALDH1 / ALDH1A1 / ALDC recombinant protein

• Host: E Coli
• Purity/Purification: > 98 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 20% glycerol, 1mM DTT, pH 8.0
• Sequence Length: 501

• Application Notes: The recombinant human ALDH1A1comprises 507 amino acids and has a predicted molecular mass of 41.5 kDa. It migrates as an approximately 45-50 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for ALDH1 / ALDH1A1 / ALDC recombinant protein

Background: Aldehyde dehydrogenase 1 family, member A1 (ALDH1A1), also known as Aldehyde dehydrogenase 1 (ALDH1), or Retinaldehyde Dehydrogenase 1 (RALDH1), is an enzyme that is expressed at high levels in stem cells and that has been suggested to regulate stem cell function. The retinaldehyde dehydrogenase (RALDH) subfamily of ALDHs, composed of ALDH1A1, ALDH1A2, ALDH1A3, and ALDH8A1, regulate development by catalyzing retinoic acid biosynthesis. The ALDH1A1 protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. ALDH1A1 also belongs to the group of corneal crystallins that help maintain the transparency of the cornea. Increased ALDH1A1 activity has been found in the stem cell populations of leukemia and some solid tumors. In tumor specimens, increased ALDH1A1 immunopositivity was found not only in secretory type cancer epithelial cells but also in neuroendocrine tumor populations. ALDH1 has been identified as a reliable marker of breast cancer stem cells. ALDH1 expression in primary cancer is an independent prognostic factor in node-positive breast cancer patients. ALDH1A1 plays a key role in normal hematopoiesis, and as a TLX1 transcriptional target, ALDH1A1 may contribute to the ability of this homeoprotein to alter cell fate and induce tumor growth.

Description: A DNA sequence encoding the human ALDH1A1 (NP_000680.2) (Ser 2-Ser 501) was expressed, with a polyhistidine tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 21361176
• NCBI GeneID: 216
• NCBI Accession #: NP_000680.2
• NCBI GenBank Nucleotide #: NM_000689.4
• UniProt Accession #: P00352; O00768; Q5SYR1
• Molecular Weight: 54,862 Da
• NCBI Official Full Name: retinal dehydrogenase 1
• NCBI Official Synonym Full Names: aldehyde dehydrogenase 1 family member A1
• NCBI Official Symbol: ALDH1A1
• NCBI Official Synonym Symbols: ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e
• NCBI Protein Information: retinal dehydrogenase 1
• UniProt Protein Name: Retinal dehydrogenase 1
• UniProt Gene Name: ALDH1A1
• UniProt Synonym Gene Names: RALDH 1Curated; RalDH1Curated

NCBI Description

The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. [provided by RefSeq, Mar 2011]

Uniprot Description

ALDH1A1: Binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. Belongs to the aldehyde dehydrogenase family.

Protein type: Apoptosis; Cofactor and Vitamin Metabolism - retinol; EC 1.2.1.36; GAPs; GAPs, Ras; Oxidoreductase

Chromosomal Location of Human Ortholog: 9q21.13

Cellular Component: cytoplasm; cytosol

Molecular Function: aldehyde dehydrogenase (NAD) activity; androgen binding; benzaldehyde dehydrogenase (NAD+) activity; GTPase activator activity; retinal dehydrogenase activity

Biological Process: aldehyde metabolic process; ethanol oxidation; retinoid metabolic process