CLPS / Colipase Recombinant Protein | CLPS recombinant protein

Recombinant Human CLPS / Colipase Protein (His tag)

Cat. Num. AAA2545275

• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: CLPS / Colipase; Recombinant Human CLPS / Colipase Protein (His tag); CLPS; CLPS recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 500mM NaCl, pH 7.0, 10% gly
• Sequence Length: 112

• Application Notes: The recombinant human CLPS consists of 105 amino acids and predicts a molecular mass of 11.5 kDa. It migrates as an approximately 12 KDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Ala 18
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CLPS recombinant protein

Background: Colipase belongs to the colipase family. Structural studies of the complex and of colipase alone have revealed the functionality of its architecture. It is a small protein with five conserved disulphide bonds. Structural analogies have been recognised between a developmental protein, the pancreatic lipase C-terminal domain, the N-terminal domains of lipoxygenases and the C-terminal domain of alpha-toxin. Colipase can only be detected in pancreatic acinar cells, suggesting regulation of expression by tissue-specific elements. Colipase allows lipase to anchor noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. Without colipase the enzyme is washed off by bile salts, which have an inhibitory effect on the lipasE Colipase is a cofactor needed by pancreatic lipase for efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilising as active conformation and considerably increasing the overall hydrophobic binding site.

Description: A DNA sequence encoding the human CLPS (P04118) (Met 1-Gln 112) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4502895
• NCBI GeneID: 1208
• NCBI Accession #: NP_001823.1
• NCBI GenBank Nucleotide #: NM_001832.3
• UniProt Accession #: P04118; Q5T9G7; Q5U809
• Molecular Weight: 11,954 Da
• NCBI Official Full Name: colipase isoform 1 preproprotein
• NCBI Official Synonym Full Names: colipase
• NCBI Official Symbol: CLPS
• NCBI Protein Information: colipase
• UniProt Protein Name: Colipase
• Protein Family: Colipase
• UniProt Gene Name: CLPS

NCBI Description

The protein encoded by this gene is a cofactor needed by pancreatic lipase for efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilizing an active conformation and considerably increasing the overall hydrophobic binding site. The gene product allows lipase to anchor noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. This cofactor is only expressed in pancreatic acinar cells, suggesting regulation of expression by tissue-specific elements. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

CLPS: Colipase is a cofactor of pancreatic lipase. It allows the lipase to anchor itself to the lipid-water interface. Without colipase the enzyme is washed off by bile salts, which have an inhibitory effect on the lipase. Belongs to the colipase family.

Protein type: Activator; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p21.31

Cellular Component: extracellular region

Molecular Function: enzyme activator activity

Biological Process: lipid digestion; response to food; retinoid metabolic process