DPP4 / DPPIV / CD26 active protein

Recombinant Human DPP4 / DPPIV / CD26 Protein (Fc tag)

Cat. Num. AAA2545154

• Gene Names: DPP4; CD26; ADABP; ADCP2; DPPIV; TP103
• Reactivity: Human
• Purity: >95 % as determined by reducing SDS-PAGE.
• Synonyms: DPP4 / DPPIV / CD26; Recombinant Human DPP4 / DPPIV / CD26 Protein (Fc tag); DPP4; ADABP; ADCP2; CD26; DPPIV; TP103; Dipeptidyl peptidase 4; Adenosine deaminase complexing protein 2; ADCP-2; Dipeptidyl peptidase IV; DPP IV; T-cell activation antigen CD26; DPP4 / DPPIV / CD26 active protein

• Host: HEK293 Cells
• Reactivity: Human
• Purity/Purification: >95 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.
• Sequence: Asn 29-Pro 766

• Application Notes: The recombinant human Fc/DPPIV is a disulfide-linked homodimeric protein.The reduced monomer consists of 975 amino acids and has a predicted molecular mass of 112 kDa. As a result of glycosylation, the apparent molecular mass of rhFc/DPPIV monomer is approximately 120-130 kDa in SDS-PAGE under reducing conditions.
• Tag: N-Fc
• Bio activity: 1. Measured by its ability to bind recombinant Cynomolgus CXCL12 in a functionalELISA.; 2. Measured by its ability to bind recombinant Human SDF1b in a functionalELISA.; 3. Using the Octet RED System, the affinity constant (Kd) of human Fc-DPPIV bound to Spike (HCoV-EMC/2012) was 11 nM.; 4. Using the Octet RED System, the affinity constant (Kd) of human Fc-DPPIV bound to Spike (HCoV-EMC/2012) was 32 nM.; 5. Using the Octet RED System, the affinity constant (Kd) of human Fc-DPPIV bound to Spike (HCoV-EMC/2012) (ECD, aa 1-1297) was 43 nM.; 6. Using the Octet RED System, the affinity constant (Kd) of human Fc-DPPIV bound to Spike-His (aa 1-760) was 12 nM.
• Endotoxin: < 1.0 EU per µg as determined by the LAL method.
• Reconstitution: It is recommended that sterile water(80 ul) be added to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Lyophilized protein should be stored at < -20°C. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

SDS-PAGE

Related Product Information for DPP4 / DPPIV / CD26 active protein

Background: Dipeptidyl peptidase-4 (DPP4) or adenosine deaminase complexing protein 2 (ADCP 2) or T-cell activation antigen CD26 is a serine exopeptidase belonging to the S9B protein family that cleaves X-proline dipeptides from the N-terminus of polypeptides, such as chemokines, neuropeptides, and peptide hormones. The enzyme is a type II transmembrane glycoprotein, expressed on the surface of many cell type s. It is also present in serum and other body fluids in a truncated form (sCD26/DPPIV). The soluble CD26 (sCD26) as a tumour marker for the detection of colorectal cancer (CRC) and advanced adenomas. As both a regulatory enzyme and a signalling factor, DPP4 has been evaluated and described in many studies. DPP4 inhibition results in increased blood concentration of the incretin hormones glucagon-like peptide-1 (GLP-1) and gastric inhibitory polypeptide (GIP). This causes an increase in glucose-dependent stimulation, resulting in a lowering of blood glucose levels. Recent studies have shown that DPP4 inhibitors can induce a significant reduction in glycosylated haemoglobin (HbA (1c)) levels, either as monotherapy or as a combination with other antidiabetic agents. Research has also demonstrated that DPP4 inhibitors portray a very low risk of hypoglycaemia development, and are a new pharmacological class of drugs for treating type 2 diabetes.

Description: A DNA sequence encoding the extracellular domain (Asn 29-Pro 766) of the mature form of human DPPIV (NP_001926.2) was expressed with the fused Fc region of human IgG1 at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 18765694
• NCBI GeneID: 1803
• NCBI Accession #: NP_001926.2
• NCBI GenBank Nucleotide #: NM_001935.3
• UniProt Accession #: P27487; Q53TN1
• Molecular Weight: Mol Mass: 112 kDa; AP Mol Mass: 120-130 kDa
• NCBI Official Full Name: dipeptidyl peptidase 4
• NCBI Official Synonym Full Names: dipeptidyl peptidase 4
• NCBI Official Symbol: DPP4
• NCBI Official Synonym Symbols: CD26; ADABP; ADCP2; DPPIV; TP103
• NCBI Protein Information: dipeptidyl peptidase 4
• UniProt Protein Name: Dipeptidyl peptidase 4
• UniProt Gene Name: DPP4
• UniProt Synonym Gene Names: ADCP2; CD26; ADCP-2; DPP IV

NCBI Description

The protein encoded by this gene is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. [provided by RefSeq, Jul 2008]

Uniprot Description

DPP4: Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF- kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. Belongs to the peptidase S9B family. DPPIV subfamily.

Protein type: Cell adhesion; Cell surface; EC 3.4.14.5; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 2q24.2

Cellular Component: apical plasma membrane; cell surface; endocytic vesicle; focal adhesion; lamellipodium; lysosomal membrane; membrane; plasma membrane

Molecular Function: dipeptidyl-peptidase activity; identical protein binding; protease binding; protein binding; protein homodimerization activity; receptor binding; serine-type peptidase activity; viral receptor activity

Biological Process: endothelial cell migration; positive regulation of cell proliferation; regulation of cell-cell adhesion mediated by integrin; response to hypoxia; T cell activation; T cell costimulation