MKK6 / MEK6 / MAP2K6 recombinant protein

Recombinant Human MKK6 / MEK6 / MAP2K6 Protein (His & GST tag)

Cat. Num. AAA2545115

• Gene Names: MAP2K6; MEK6; MKK6; MAPKK6; PRKMK6; SAPKK3; SAPKK-3
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: MKK6 / MEK6 / MAP2K6; Recombinant Human MKK6 / MEK6 / MAP2K6 Protein (His & GST tag); MAP2K6; MAPKK6; MEK6; MKK6; PRKMK6; SAPKK3; MKK6 / MEK6 / MAP2K6 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0
• Sequence Length: 334

• Application Notes: The recombinant human MKK6/GST chimera consists of 571 amino acids and has a calculated molecular mass of 65.3 kDa. It migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for MKK6 / MEK6 / MAP2K6 recombinant protein

Background: Dual specificity mitogen-activated protein kinase kinase 6, also known as MAP kinase kinase 6, MAPKK 6, MAPK / ERK kinase 6, SAPKK3, MAP2K6 and MKK6, is a protein which belongs to the protein kinase superfamily, STE Ser / Thr protein kinase family and MAP kinase kinase subfamily. MAP2K6 / MKK6 contains one protein kinase domain. Mitogen-activated protein kinases are members of a conserved cascade of kinases involved in many signal transduction pathways. They stimulate phosphorylation of transcription factors in response to extracellular signals such as growth factors, cytokines, ultraviolet light, and stress-inducing agents. MAP2K6 / MKK6 exists in a variety of alternatively spliced isoforms with distinct patterns of tissue expression. Isoform 2 of MAP2K6 / MKK6 is only expressed in skeletal muscle. Isoform 1 of MAP2K6 / MKK6 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.

Description: A DNA sequence encoding the human MKK6 isoform 1 (P52564-1) (Met 1-Asp 334) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 14589900
• NCBI GeneID: 5608
• NCBI Accession #: NP_002749.2
• NCBI GenBank Nucleotide #: NM_002758.3
• UniProt Accession #: P52564
• Molecular Weight: 31,339 Da
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 6 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 6
• NCBI Official Symbol: MAP2K6
• NCBI Official Synonym Symbols: MEK6; MKK6; MAPKK6; PRKMK6; SAPKK3; SAPKK-3
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 6
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 6
• UniProt Gene Name: MAP2K6
• UniProt Synonym Gene Names: MEK6; MKK6; PRKMK6; SKK3; MAP kinase kinase 6; MAPKK 6; MEK 6; SAPK kinase 3; SAPKK-3; SAPKK3

NCBI Description

This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis. [provided by RefSeq, Jul 2008]

Uniprot Description

MKK6: a dual-specificity protein kinase of the STE7 family. Activates p38 MAP kinase by phosphorylating a Thr and a Tyr residue in the activation loop. Is activated by cytokines and environmental stress in vivo. Three alternatively spliced isoforms have been reported.

Protein type: EC 2.7.12.2; Kinase, protein; Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); STE group; STE7 family

Chromosomal Location of Human Ortholog: 17q24.3

Cellular Component: cytoplasm; cytosol; nucleoplasm

Molecular Function: MAP kinase kinase activity; protein binding; protein kinase binding; receptor signaling protein serine/threonine kinase activity

Biological Process: activation of MAPK activity; cell cycle arrest; DNA damage induced protein phosphorylation; signal transduction