TAOK3 / JIK / MAP3K18 recombinant protein

Recombinant Human TAOK3 / JIK / MAP3K18 Protein (aa 1-411, His & GST tag)

Cat. Num. AAA2545064

• Gene Names: TAOK3; DPK; JIK; hKFC-A; MAP3K18
• Purity: > 92 % as determined by SDS-PAGE
• Synonyms: TAOK3 / JIK / MAP3K18; Recombinant Human TAOK3 / JIK / MAP3K18 Protein (aa 1-411; His & GST tag); DKFZp666H245; DPK; FLJ31808; JIK; MAP3K18; TAOK3 / JIK / MAP3K18 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 92 % as determined by SDS-PAGE
• Form/Format: Supplied as sterile 20mM Tris, 500mM NaCl, 3mM GSH, pH 7.4, 10% gly
• Sequence Length: 898

• Application Notes: The recombinant human TAOK3 (aa 1-411)/GST chimera consists of 648 amino acids and has a calculated molecular mass of 74.3 kDa. It migrates as an approximately 85 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for TAOK3 / JIK / MAP3K18 recombinant protein

Background: Serine/threonine-protein kinase TAO3, also known as cutaneous T-cell lymphoma-associated antigen HD-CL-09, CTCL-associated antigen HD-CL-09, Dendritic cell-derived protein kinase, JNK / SAPK-inhibitory kinase, Jun kinase-inhibitory kinase, Kinase from chicken homolog A, Thousand and one amino acid protein 3, JIK, TAOK3 and MAP3K18, is cytoplasm and peripheral membrane protein which belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. Protein kinases are pivotal regulators of cell signaling that modulate each other's functions and activities through site-specific phosphorylation events. TAOK3 / JIK contains one protein kinase domain. TAOK3 / JIK is ubiquitously expressed at a low level, and highly expressed in peripheral blood leukocytes (PBLs), thymus, spleen, kidney, skeletal muscle, heart and liver. TAOK3 / JIK inhibits the basal activity of Jun kinase. It is negatively regulated by epidermal growth factor (EGF). When overexpressed, TAOK3 / JIK may activate ERK1 / ERK2 and JNK / SAPK.

Description: A DNA sequence encoding the amino acids (Met 1-Asp 411) of human TAOK3 (Q9H2K8-1) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 1080078670
• NCBI GeneID: 51347
• NCBI Accession #: NP_001333417.1
• NCBI GenBank Nucleotide #: NM_001346488.1
• UniProt Accession #: Q9H2K8; Q658N1; Q8IUM4; Q9HC79; Q9NZM9; Q9UHG7
• Molecular Weight: 105,406 Da
• NCBI Official Full Name: serine/threonine-protein kinase TAO3 isoform b
• NCBI Official Synonym Full Names: TAO kinase 3
• NCBI Official Symbol: TAOK3
• NCBI Official Synonym Symbols: DPK; JIK; hKFC-A; MAP3K18
• NCBI Protein Information: serine/threonine-protein kinase TAO3
• UniProt Protein Name: Serine/threonine-protein kinase TAO3
• UniProt Gene Name: TAOK3
• UniProt Synonym Gene Names: DPK; JIK; KDS; MAP3K18; CTCL-associated antigen HD-CL-09; hKFC-A

NCBI Description

The protein encoded by this gene is a serine/threonine protein kinase that activates the p38/MAPK14 stress-activated MAPK cascade but inhibits the basal activity of the MAPK8/JNK cascade. The encoded protein is a member of the GCK subfamily of STE20-like kinases. [provided by RefSeq, Oct 2016]

Uniprot Description

TAO3: Serine/threonine-protein kinase that acts as a regulator of the p38/MAPK14 stress-activated MAPK cascade and of the MAPK8/JNK cascade. Acts as an activator of the p38/MAPK14 stress- activated MAPK cascade. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. Inhibits basal activity of MAPK8/JNK cascade and diminishes its activation in response epidermal growth factor (EGF). Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; TAO subfamily

Chromosomal Location of Human Ortholog: 12q24.23

Cellular Component: cytoplasm

Molecular Function: protein binding; protein kinase inhibitor activity; protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity; transferase activity

Biological Process: MAPKKK cascade; mitotic cell cycle G2/M transition DNA damage checkpoint; negative regulation of JNK cascade; positive regulation of JNK activity; positive regulation of JNK cascade; positive regulation of stress-activated MAPK cascade; protein amino acid autophosphorylation; protein amino acid phosphorylation; response to DNA damage stimulus