Rabbit anti-Mouse ECM1  Monoclonal Antibody | anti-ECM1  antibody

Rabbit anti Mouse ECM1  Monoclonal Antibody

Cat. Num. AAA2544553

• Gene Names: ECM1; URBWD
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: ECM1 ; Monoclonal Antibody; Rabbit anti Mouse ECM1 Monoclonal Antibody; anti-ECM1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 415

• Applicable Applications for anti-ECM1 antibody: ELISA (EIA)
• Application Notes: ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Mouse ECM1. The detection limit for Mouse ECM1 is approximately 0.039 ng/well.
• Immunogen: Recombinant Mouse ECM1 protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-ECM1  antibody

Extracellular matrix protein 1 (ECM1) is a secreted glycoprotein and playing a pivotal role in endochondral bone formation, angiogenesis, and tumour biology. Three splice variants have been identified: ECM1a (540 aa) is most widely expressed, with highest expression in the placenta and heart; ECM1b (415 aa) is differentiation-dependent expressed and found only in tonsil and associated with suprabasal keratinocytes; ECM1c (559 aa) accounts for approximately 15% of skin ECM1. Although ECM1 is not tumor specific, is significantly elevated in many malignant epithelial tumors and is suggested as a possible trigger for angiogenesis, tumor progression and malignancies. It also has been shown to regulate endochondral bone formation, skeletal development and tissue remodeling. 

NCBI and Uniprot Product Information

• NCBI GI #: 221316616
• NCBI GeneID: 1893
• NCBI GenBank Nucleotide #: NM_022664.2
• Molecular Weight: 63,563 Da
• NCBI Official Full Name: extracellular matrix protein 1 isoform 2
• NCBI Official Synonym Full Names: extracellular matrix protein 1
• NCBI Official Symbol: ECM1
• NCBI Official Synonym Symbols: URBWD
• NCBI Protein Information: extracellular matrix protein 1
• UniProt Protein Name: Extracellular matrix protein 1
• UniProt Gene Name: ECM1
• UniProt Entry Name: ECM1_HUMAN

NCBI Description

This gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

ECM1: Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity. Defects in ECM1 are the cause of lipoid proteinosis (LiP); also known as lipoid proteinosis of Urbach and Wiethe or hyalinosis cutis et mucosae. LiP is a rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular matrix; extracellular region

Molecular Function: enzyme binding; laminin binding; protease binding; protein binding; protein C-terminus binding; signal transducer activity

Biological Process: negative regulation of bone mineralization; negative regulation of peptidase activity; platelet degranulation; positive regulation of angiogenesis; positive regulation of endothelial cell proliferation; positive regulation of I-kappaB kinase/NF-kappaB cascade

Disease: Lipoid Proteinosis Of Urbach And Wiethe