Rabbit anti-Human CD97  Monoclonal Antibody | anti-CD97  antibody

Rabbit anti Human CD97  Monoclonal Antibody

Cat. Num. AAA2544220

• Gene Names: ADGRE5; CD97; TM7LN1
• Reactivity: Human
• Applications: ELISA, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: CD97 ; Monoclonal Antibody; Rabbit anti Human CD97 Monoclonal Antibody; anti-CD97 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 835

• Applicable Applications for anti-CD97 antibody: ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Human CD97. The detection limit for Human CD97 is approximately 0.0049 ng/well.; Flow Cytometry: 0.5-2 mug/Test; ICC/IF: 20-30 mug/mL
• Immunogen: Recombinant Human CD97 protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

NCBI and Uniprot Product Information

• NCBI GI #: 17978491
• NCBI GeneID: 976
• NCBI GenBank Nucleotide #: NM_078481.3
• Molecular Weight: 86,628 Da
• NCBI Official Full Name: CD97 antigen isoform 1 preproprotein
• NCBI Official Synonym Full Names: adhesion G protein-coupled receptor E5
• NCBI Official Symbol: ADGRE5
• NCBI Official Synonym Symbols: CD97; TM7LN1
• NCBI Protein Information: CD97 antigen
• UniProt Protein Name: CD97 antigen
• UniProt Gene Name: CD97
• UniProt Entry Name: CD97_HUMAN

NCBI Description

This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. [provided by RefSeq, Jun 2011]

Uniprot Description

Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration ().