Rabbit anti-Human CD40L/CD40LG  Monoclonal Antibody | anti-CD40L/CD40LG  antibody

Rabbit anti Human CD40L/CD40LG  Monoclonal Antibody

Cat. Num. AAA2543895

• Gene Names: CD40LG; IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
• Reactivity: Human
• Applications: ELISA
• Synonyms: CD40L/CD40LG ; Monoclonal Antibody; Rabbit anti Human CD40L/CD40LG Monoclonal Antibody; anti-CD40L/CD40LG antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 261

• Applicable Applications for anti-CD40L/CD40LG antibody: ELISA (EIA)
• Application Notes: ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Human CD40L. The detection limit for Human CD40L is approximately 0.00975 ng/well.
• Immunogen: Recombinant Human CD40L/CD154/TNFSF5 protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-CD40L/CD40LG  antibody

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD154, also known as CD40 ligand or CD40L, is a member of the TNF superfamily. While CD154 was originally found on T cell surface, its expression has since been found on a wide variety of cells, including platelets, mast cells, macrophages and NK cells. CD154's ability is achieved through binding to the CD40 on antigen- presenting cells (APC). In the macrophage cells, the primary signal for activation is IFN-gamma from Th1 type CD4 T cells. The secondary signal is CD40L on the T cell, which interacting with the CD40 molecules, helping increase the level of activation.

NCBI and Uniprot Product Information

• NCBI GI #: 4557433
• NCBI GeneID: 959
• NCBI GenBank Nucleotide #: NM_000074.2
• Molecular Weight: 29,274 Da
• NCBI Official Full Name: CD40 ligand
• NCBI Official Synonym Full Names: CD40 ligand
• NCBI Official Symbol: CD40LG
• NCBI Official Synonym Symbols: IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
• NCBI Protein Information: CD40 ligand
• UniProt Protein Name: CD40 ligand
• UniProt Gene Name: CD40LG
• UniProt Synonym Gene Names: CD40L; TNFSF5; TRAP; CD40-L; TRAP
• UniProt Entry Name: CD40L_HUMAN

NCBI Description

The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

CD40LG: Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1); also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. Belongs to the tumor necrosis factor family.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq26

Cellular Component: cell surface; integral to plasma membrane; plasma membrane

Molecular Function: CD40 receptor binding; protein binding

Biological Process: activation of JNK activity; activation of NF-kappaB transcription factor; B cell proliferation; inflammatory response; isotype switching; negative regulation of apoptosis; platelet activation; positive regulation of interleukin-10 production; positive regulation of interleukin-12 production; positive regulation of interleukin-4 production; positive regulation of T cell proliferation; regulation of immune response; T cell costimulation; tumor necrosis factor-mediated signaling pathway

Disease: Immunodeficiency With Hyper-igm, Type 1