Rabbit anti-Human IL18  Monoclonal Antibody | anti-IL18  antibody

Rabbit anti Human IL18  Monoclonal Antibody

Cat. Num. AAA2543849

• Gene Names: IL18; IGIF; IL-18; IL-1g; IL1F4
• Reactivity: Human
• Applications: ELISA
• Synonyms: IL18 ; Monoclonal Antibody; Rabbit anti Human IL18 Monoclonal Antibody; anti-IL18 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 193

• Applicable Applications for anti-IL18 antibody: ELISA (EIA)
• Application Notes: ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Human IL18. The detection limit for Human IL18 is approximately 0.078 ng/well.
• Immunogen: Recombinant Human IL-18 protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-IL18  antibody

Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1-like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cells-, IL-4- and STAT6-dependent fashion. IL-18 and T cell receptor-mediated stimulation could induce naïve CD4+ T cells to develop into IL-4-producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-alpha and IL-1beta, suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.

NCBI and Uniprot Product Information

• NCBI GI #: 4504653
• NCBI GeneID: 3606
• NCBI GenBank Nucleotide #: NM_001562.3
• Molecular Weight: 21,896 Da
• NCBI Official Full Name: interleukin-18 isoform 1
• NCBI Official Synonym Full Names: interleukin 18
• NCBI Official Symbol: IL18
• NCBI Official Synonym Symbols: IGIF; IL-18; IL-1g; IL1F4
• NCBI Protein Information: interleukin-18
• UniProt Protein Name: Interleukin-18
• UniProt Gene Name: IL18
• UniProt Synonym Gene Names: IGIF; IL1F4; IL-18; IFN-gamma-inducing factor; IL-1 gamma
• UniProt Entry Name: IL18_HUMAN

NCBI Description

The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]

Uniprot Description

IL18: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. Belongs to the IL-1 family.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 11q22.2-q22.3

Cellular Component: cytosol; extracellular region; extracellular space

Molecular Function: cytokine activity; protein binding

Biological Process: activation of protein kinase B; angiogenesis; cell-cell signaling; chemokine biosynthetic process; cholesterol homeostasis; granulocyte macrophage colony-stimulating factor biosynthetic process; immune response; inflammatory response; interferon-gamma biosynthetic process; interleukin-13 biosynthetic process; interleukin-2 biosynthetic process; lipopolysaccharide-mediated signaling pathway; MAPKKK cascade; positive regulation of activated T cell proliferation; positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of inflammatory response; positive regulation of interferon-gamma production; positive regulation of interleukin-17 production; positive regulation of natural killer cell proliferation; positive regulation of NK T cell proliferation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of smooth muscle cell proliferation; positive regulation of T-helper 2 cell differentiation; positive regulation of tissue remodeling; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of Stat3 protein; regulation of cell adhesion; sleep; T-helper 1 type immune response; T-helper 2 type immune response