Rabbit anti-Human 4E-BP1/EIF4EBP1  Monoclonal Antibody | anti-4E-BP1/EIF4EBP1  antibody

Rabbit anti Human 4E-BP1/EIF4EBP1  Monoclonal Antibody

Cat. Num. AAA2543810

• Reactivity: Human
• Applications: Western Blot, ELISA
• Synonyms: 4E-BP1/EIF4EBP1 ; Monoclonal Antibody; Rabbit anti Human 4E-BP1/EIF4EBP1 Monoclonal Antibody; anti-4E-BP1/EIF4EBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG

• Applicable Applications for anti-4E-BP1/EIF4EBP1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 0.1-1 mug/mL; ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Human 4EBP1. The detection limit for Human 4EBP1 is approximately 0.00975 ng/well.
• Immunogen: Recombinant Human 4E-BP1/EIF4EBP1 protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-4E-BP1/EIF4EBP1  antibody

The translational suppressor eIF4E binding protein-1, 4E-BP1 functions as a key regulator in cellular growth, differentiation, apoptosis and survival. The Eif4ebp1 gene, encoding 4E-BP1, is a direct target of a transcription factor activating transcription factor-4 (ATF4), a master regulator of gene expression in stress responses. 4E-BP1 is characterized by its capacity to bind specifically to eIF4E and inhibit its interaction with eIF4G. Phosphorylation of 4E-BP1 regulates eIF4E availability, and therefore, cap-dependent translation, in cell stress. Binding of eIF4E to eIF4G is inhibited in a competitive manner by 4E-BP1. Phosphorylation of 4E-BP1 decreases the affinity of this protein for eIF4E, thus favouring the binding of eIF4G and enhancing translation. 4E-BP1 is important for beta-cell survival under endoplasmic reticulum (ER) stress. 4E-BP1 mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. Recently, 4E-BP1 was found to be a key factor, which converges several oncogenic signals, phosphorylates the molecules, and drives the downstream proliferative signals. Recent studies showed that high expression of phosphorylated 4E-BP-1 (p-4E-BP1) is associated with poor prognosis, tumor progression, or nodal metastasis in different human cancers.