Mouse anti-Human Caspase-14/CASP14 Monoclonal Antibody | anti-CASP14 antibody

Mouse anti Human Caspase-14/CASP14 Monoclonal Antibody

Cat. Num. AAA2543657

• Reactivity: Human
• Applications: ELISA
• Synonyms: Caspase-14/CASP14; Monoclonal Antibody; Mouse anti Human Caspase-14/CASP14 Monoclonal Antibody; anti-CASP14 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Sequence Length: 242

• Applicable Applications for anti-CASP14 antibody: ELISA (EIA)
• Application Notes: ELISA: 0.5-1 mug/mL. This antibody can be used at 0.5-1 mug/mL with the appropriate secondary reagents to detect Human CASP14. The detection limit for Human CASP14 is approximately 0.16 ng/well.
• Immunogen: Recombinant Human Caspase-14/CASP14 protein
• Buffer: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Caspase-14/CASP14 (rh Caspase-14/CASP14; NP_036246.1; Ser 2-Gln 242). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -70 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

NCBI and Uniprot Product Information

• NCBI GI #: 169666460
• NCBI GeneID: 23581
• Molecular Weight: 27,680 Da
• NCBI Official Full Name: caspase-14
• NCBI Official Synonym Full Names: caspase 14
• NCBI Official Symbol: CASP14
• NCBI Protein Information: caspase-14
• UniProt Protein Name: Caspase-14
• Protein Family: Caspase
• UniProt Gene Name: CASP14
• UniProt Synonym Gene Names: CASP-14
• UniProt Entry Name: CASPE_HUMAN

NCBI Description

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This caspase has been shown to be processed and activated by caspase 8 and caspase 10 in vitro, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier. [provided by RefSeq, Jul 2008]

Uniprot Description

CASP14: Believed to be a non-apoptotic caspase which is involved in epidermal differentiation. Seems to play a role in keratinocyte differentiation and cornification. Probably regulates maturation of the epidermis by proteolytically processing filaggrin. Complex of unprocessed caspase-14 and processed 19 kDa (p19) and 10 kDa (p10) subunits. In undifferentiated keratinocytes under postconfluency growth conditions (in vitro). Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum). Expressed in keratinocytes of hair shaft and sebaceous glands. In psoriatic skin only expressed at very low levels. Belongs to the peptidase C14A family.

Protein type: EC 3.4.22.-; Protease

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: cytoplasm; extracellular matrix; nucleus

Molecular Function: cysteine-type endopeptidase activity; protein binding

Biological Process: epidermis development; keratinization