Mouse anti-Human Cadherin-12/CDH12 Monoclonal Antibody | anti-CDH12 antibody

Mouse anti Human Cadherin-12/CDH12 Monoclonal Antibody

Cat. Num. AAA2543320

• Gene Names: CDH12; CDHB
• Reactivity: Human
• Applications: ELISA
• Synonyms: Cadherin-12/CDH12; Monoclonal Antibody; Mouse anti Human Cadherin-12/CDH12 Monoclonal Antibody; anti-CDH12 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Sequence Length: 794

• Applicable Applications for anti-CDH12 antibody: ELISA (EIA)
• Application Notes: ELISA: 0.5-1 mug/mL. This antibody can be used at 0.5-1 mug/mL with the appropriate secondary reagents to detect Human CDH12. The detection limit for Human CDH12 is approximately 0.078 ng/well.
• Immunogen: Recombinant Human Cadherin-12/CDH12 Protein
• Buffer: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Cadherin-12/CDH12 (rh Cadherin-12/CDH12; NP_001783.2; Met 1-Ala 605). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -70 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

NCBI and Uniprot Product Information

• NCBI GI #: 954170028
• NCBI GeneID: 1010
• NCBI GenBank Nucleotide #: NM_001317227.1
• Molecular Weight: 84,001 Da
• NCBI Official Full Name: cadherin-12 isoform 1 preproprotein
• NCBI Official Synonym Full Names: cadherin 12
• NCBI Official Symbol: CDH12
• NCBI Official Synonym Symbols: CDHB
• NCBI Protein Information: cadherin-12
• UniProt Protein Name: Cadherin-12
• Protein Family: Cadherin
• UniProt Gene Name: CDH12
• UniProt Synonym Gene Names: BR-cadherin; N-cadherin 2
• UniProt Entry Name: CAD12_HUMAN

NCBI Description

This gene encodes a type II classical cadherin of the cadherin superfamily. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature cadherin protein. These integral membrane proteins mediate calcium-dependent cell-cell adhesion and are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a histidine-alanine-valine (HAV) cell adhesion recognition sequence specific to type I cadherins. This particular cadherin appears to be expressed specifically in the brain and its temporal pattern of expression would be consistent with a role during a critical period of neuronal development, perhaps specifically during synaptogenesis. [provided by RefSeq, Nov 2015]

Uniprot Description

CDH12: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5p14.3

Cellular Component: plasma membrane