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Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded Human breast cancer using RPA2 Polyclonal Antibody at dilution of 1/45)

Rabbit anti-Human RPA2 Polyclonal Antibody | anti-RPA2 antibody

RPA2 Polyclonal Antibody

Gene Names
RPA2; REPA2; RPA32; RP-A p32; RP-A p34
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purification
Synonyms
RPA2; Polyclonal Antibody; RPA2 Polyclonal Antibody; REPA2; RPA32; RP-A p32; RP-A p34; anti-RPA2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purification
Concentration
2.6mg/ml (varies by lot)
Sequence Length
270
Applicable Applications for anti-RPA2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
ELISA: 1:2000-5000
IHC: 1:30-150
WB: 1:500-2000
Immunogen
Full length fusion protein
Buffer
PBS with 0.05% sodium azide, 50% glycerol, pH7.3
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-46501 / sc-46626 / sc-14691 / sc-14692 / sc-28709
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human breast cancer using RPA2 Polyclonal Antibody at dilution of 1/45)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded Human breast cancer using RPA2 Polyclonal Antibody at dilution of 1/45)

Western Blot (WB)

(Western Blot analysis of Hela, huvec, HepG2, A549, Jurkat, 231 and A431 cells using RPA2 Polyclonal Antibody at dilution of 1/650)

Western Blot (WB) (Western Blot analysis of Hela, huvec, HepG2, A549, Jurkat, 231 and A431 cells using RPA2 Polyclonal Antibody at dilution of 1/650)
Related Product Information for anti-RPA2 antibody
Replication protein A 32 kDa subunit is a protein that in humans is encoded by theRPA2 gene. As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
29 kDa
NCBI Official Full Name
RPA2, partial
NCBI Official Synonym Full Names
replication protein A2
NCBI Official Symbol
RPA2
NCBI Official Synonym Symbols
REPA2; RPA32; RP-A p32; RP-A p34
NCBI Protein Information
replication protein A 32 kDa subunit
UniProt Protein Name
Replication protein A 32 kDa subunit
Protein Family
UniProt Gene Name
RPA2
UniProt Synonym Gene Names
REPA2; RPA32; RPA34; RP-A p32; RF-A protein 2; RP-A p34
UniProt Entry Name
RFA2_HUMAN

Uniprot Description

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.

Research Articles on RPA2

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Product Notes

The RPA2 rpa2 (Catalog #AAA2542411) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RPA2 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RPA2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). ELISA: 1:2000-5000 IHC: 1:30-150 WB: 1:500-2000. Researchers should empirically determine the suitability of the RPA2 rpa2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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