Rabbit anti-Human, Mouse Laminin alpha-5 Polyclonal Antibody | anti-LAMA5 antibody

Laminin alpha-5 Polyclonal Antibody

Cat. Num. AAA2535681

• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity purification
• Synonyms: Laminin alpha-5; Polyclonal Antibody; Laminin alpha-5 Polyclonal Antibody; LAMA5; KIAA0533; KIAA1907; Laminin subunit alpha-5; Laminin-10 subunit alpha; Laminin-11 subunit alpha; Laminin-15 subunit alpha; anti-LAMA5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4

• Applicable Applications for anti-LAMA5 antibody: Western Blot (WB), Immunohistochemistry-paraffin (IHC-p), ELISA (EIA)
• Application Notes: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000
• Immunogen: Synthesized peptide derived from the Internal region of human Laminin α-5
• Note: Centrifuge before opening to ensure complete recovery of vial contents.
• Preparation and Storage: Store at -20°C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of RAW264.7, HepG2, A549 cells with Laminin alpha5 Polyclonal Antibody)

Related Product Information for anti-LAMA5 antibody

Components of the extracellular matrix exert myriad effects on tissues throughout the body. In particular, the laminins, a family of heterotrimeric extracellular glycoproteins, affect tissue development and integrity in such diverse organs as the kidney, lung, skin, and nervous system. It is thought that laminins mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminins function as heterotrimeric complexes of alpha, beta, and gamma chains, with each chain type representing a different subfamily of proteins. The protein encoded by this gene belongs to the alpha subfamily of laminin chains and is a major component of basement membranes. Two transcript variants encoding different isoforms have been found for this gene, but the fulllength nature of one of them has not been determined.

NCBI and Uniprot Product Information

• NCBI GI #: 21264602
• NCBI GeneID: 3911
• NCBI Accession #: NP_005551.3
• NCBI GenBank Nucleotide #: NM_005560.4
• UniProt Accession #: O15230; Q5U4N9; Q8TDF8; Q8WZA7; Q9H1P1
• Molecular Weight: Calculated MW: 400kDa; Observed MW: 400kDa
• NCBI Official Full Name: laminin subunit alpha-5
• NCBI Official Synonym Full Names: laminin subunit alpha 5
• NCBI Official Symbol: LAMA5
• NCBI Protein Information: laminin subunit alpha-5
• UniProt Protein Name: Laminin subunit alpha-5
• Protein Family: Laminin
• UniProt Gene Name: LAMA5
• UniProt Synonym Gene Names: KIAA0533; KIAA1907
• UniProt Entry Name: LAMA5_HUMAN

NCBI Description

This gene encodes one of the vertebrate laminin alpha chains. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. The protein encoded by this gene is the alpha-5 subunit of of laminin-10 (laminin-511), laminin-11 (laminin-521) and laminin-15 (laminin-523). [provided by RefSeq, Jun 2013]

Uniprot Description

LAMA5: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Extracellular matrix; Secreted

Chromosomal Location of Human Ortholog: 20q13.2-q13.3

Cellular Component: basal lamina; basement membrane; extracellular matrix; extracellular region; extracellular space; laminin-10 complex; laminin-5 complex; nucleus

Molecular Function: integrin binding; structural molecule activity

Biological Process: angiogenesis; cell differentiation; cell migration; cell proliferation; cell recognition; cilium biogenesis; cytoskeleton organization and biogenesis; embryonic development; endothelial cell differentiation; extracellular matrix disassembly; extracellular matrix organization and biogenesis; focal adhesion formation; hair follicle development; integrin-mediated signaling pathway; lung development; morphogenesis of a polarized epithelium; morphogenesis of embryonic epithelium; muscle development; neural crest cell migration; odontogenesis of dentine-containing teeth; regulation of cell adhesion; regulation of cell migration; regulation of cell proliferation; regulation of embryonic development; ureteric bud branching