Rabbit anti-Human HLA class I Polyclonal Antibody | anti-HLA antibody

HLA Class I (HLA-B) Polyclonal Antibody

Cat. Num. AAA2522514

• Gene Names: HLA-B; AS; HLAB; SPDA1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification
• Synonyms: HLA class I; Polyclonal Antibody; HLA Class I (HLA-B) Polyclonal Antibody; AS; HLA-B-7301; HLA-B73; HLAB; HLAC; MGC111087; SPDA1; anti-HLA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

• Applicable Applications for anti-HLA antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:1000-1:4000; IHC: 1:50-200
• Immunogen: Fusion protein of HLA class I (HLA-B)
• Note: Centrifuge before opening to ensure complete recovery of vial contents.
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of A549, using HLA Class I (HLA-B) Polyclonal Antibody at dilution of 1:1000.)

Immunohischemical (IHC)

(Immunohischemical ananlysis of paraffin-embedded Human spleen tissue using HLA Class I (HLA-B) Polyclonal Antibody at dilution of 1:100.)

Related Product Information for anti-HLA antibody

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and ?2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system.

NCBI and Uniprot Product Information

• NCBI GI #: 8017397
• NCBI GeneID: 3106
• UniProt Accession #: Q96DW9; P01889; P03989; P10319; P18463; P18464; P18465; P30460; P30461; P30462; P30464
• Molecular Weight: Calculated MW: 362aa, 40 kDa; Observed MW: 40~45 kDa
• NCBI Official Full Name: HLA class I, partial
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, B
• NCBI Official Symbol: HLA-B
• NCBI Official Synonym Symbols: AS; HLAB; SPDA1
• NCBI Protein Information: major histocompatibility complex, class I, B; HLA class I histocompatibility antigen, B alpha chain; MHC Class I HLA heavy chain; MHC HLA-B cell surface glycoprotein; MHC HLA-B transmembrane glycoprotein; MHC class I antigen GN00104; MHC class I antigen HLA-B heavy chain; MHC class I antigen SHCHA; leukocyte antigen class I-B; lymphocyte antigen
• UniProt Protein Name: HLA class I
• Protein Family: HLA class I histocompatibility antigen
• UniProt Gene Name: HLA-B
• UniProt Entry Name: Q9MYI4_HUMAN

NCBI Description

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

HLAB iso7: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; Golgi apparatus; phagocytic vesicle membrane; cell surface; membrane; early endosome membrane; integral to plasma membrane; endoplasmic reticulum; plasma membrane; integral to membrane; MHC class I protein complex

Molecular Function: peptide antigen binding; chaperone binding; TAP binding; receptor binding

Biological Process: regulation of immune response; detection of bacterium; viral reproduction; regulation of interleukin-12 production; positive regulation of T cell mediated cytotoxicity; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; defense response; protection from natural killer cell mediated cytotoxicity; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent; antigen processing and presentation of peptide antigen via MHC class I; regulation of T cell anergy; regulation of interleukin-6 production; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; immune response