Human HE4 ELISA Kit | HE4 elisa kit

Human HE4(epididymal protein 4)ELISA Kit

Cat. Num. AAA2515935

• Reactivity: Human
• Synonyms: HE4; Human HE4(epididymal protein 4)ELISA Kit; HE4 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human HE4 in samples. No significant cross-reactivity or interference between Human HE4 and analogues was observed.
• Sequence Length: 124

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.78-50pmol/mL
• Sensitivity: 0.47pmol/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human HE4 were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human HE4 were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HE4 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human HE4 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HE4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HE4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HE4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human HE4. You can calculate the concentration of Human HE4 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 28894243
• Molecular Weight: 11,043 Da
• NCBI Official Full Name: HE4 protein
• UniProt Protein Name: WAP four-disulfide core domain protein 2
• UniProt Gene Name: WFDC2
• UniProt Synonym Gene Names: HE4; WAP5
• UniProt Entry Name: WFDC2_HUMAN

Uniprot Description

WFDC2: a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008]

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: extracellular space

Molecular Function: serine-type endopeptidase inhibitor activity; aspartic-type endopeptidase inhibitor activity; endopeptidase inhibitor activity; cysteine protease inhibitor activity

Biological Process: spermatogenesis; proteolysis