Monkey MUC20 ELISA Kit | MUC20 elisa kit

Monkey MUC20 (Mucin 20) ELISA Kit

Cat. Num. AAA2512094

• Gene Names: MUC20; MUC-20
• Reactivity: Monkey
• Synonyms: MUC20; Monkey MUC20 (Mucin 20) ELISA Kit; MUC20 elisa kit

• Reactivity: Monkey
• Specificity: This kit recognizes natural and recombinant Monkey MUC20. No significant cross-reactivity or interference between Monkey MUC20 and analogues was observed.
• Sequence Length: 709

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
• Preparation and Storage: Store at 4 degree C.

Related Product Information for MUC20 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey MUC20 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MUC20. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MUC20 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MUC20, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of MUC20. You can calculate the concentration of MUC20 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 541444092
• NCBI GeneID: 200958
• NCBI Accession #: NP_001269435.1
• NCBI GenBank Nucleotide #: NM_001282506.1
• UniProt Accession #: Q8N307; Q6UX97; Q76I83; Q76I85; Q86ST8; Q8NBY6; Q96KA1; Q9P2I8
• Molecular Weight: 68,308 Da
• NCBI Official Full Name: mucin-20 isoform a
• NCBI Official Synonym Full Names: mucin 20, cell surface associated
• NCBI Official Symbol: MUC20
• NCBI Official Synonym Symbols: MUC-20
• NCBI Protein Information: mucin-20; transmembrane mucin MUC20S
• UniProt Protein Name: Mucin-20
• Protein Family: Mucin
• UniProt Gene Name: MUC20
• UniProt Synonym Gene Names: KIAA1359; MUC-20
• UniProt Entry Name: MUC20_HUMAN

NCBI Description

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins secreted by many epithelial tissues to form an insoluble mucous barrier. The C-terminus of this family member associates with the multifunctional docking site of the MET proto-oncogene and suppresses activation of some downstream MET signaling cascades. The protein features a mucin tandem repeat domain that varies between two and six copies in most individuals. Multiple variants encoding different isoforms have been found for this gene. A related pseudogene, which is also located on chromosome 3, has been identified. [provided by RefSeq, Apr 2014]

Uniprot Description

MUC20: May regulate MET signaling cascade. Seems to decrease hepatocyte growth factor (HGF)-induced transient MAPK activation. Blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway. Inhibits HGF-induced proliferation of MMP1 and MMP9 expression. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: Golgi lumen; microvillus membrane; apical plasma membrane; basal plasma membrane; extracellular region

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; activation of MAPK activity; hepatocyte growth factor receptor signaling pathway; post-translational protein modification; protein homooligomerization