Sheep AGA ELISA Kit | AGA elisa kit

Sheep AGA (Anti-Glycolipid Antibody) ELISA Kit

Cat. Num. AAA2511159

• Gene Names: AGA; GA; AGU; ASRG
• Reactivity: Sheep
• Synonyms: AGA; Sheep AGA (Anti-Glycolipid Antibody) ELISA Kit; AGA elisa kit

• Reactivity: Sheep
• Specificity: This kit recognizes natural and recombinant Sheep AGA. No significant cross-reactivity or interference between Sheep AGA and analogues was observed.
• Sequence Length: 346

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 1.563-100ng/mL
• Sensitivity: Min: 0.938ng/mL; Max: 100ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AGA elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Sheep AGA concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antigen specific to AGA. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antigen. Then a biotinylated detection antigen specific for AGA and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AGA, biotinylated detection antigen and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of AGA.You can calculate the concentration of AGA in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 49456391
• NCBI GeneID: 175
• UniProt Accession #: P20933; Q4W5Q2; Q6FHN6; Q9UCK6; Q9UCK7; Q9UCK8; B2R7H2; D3DP47
• Molecular Weight: 37,208 Da
• NCBI Official Full Name: AGA, partial
• NCBI Official Synonym Full Names: aspartylglucosaminidase
• NCBI Official Symbol: AGA
• NCBI Official Synonym Symbols: GA; AGU; ASRG
• NCBI Protein Information: N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase; N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase; N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase; aspartylglucosylamine deaspartylase; glycosylasparaginase
• UniProt Protein Name: N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase
• Protein Family: Agaricus bisporus lectin
• UniProt Gene Name: AGA
• UniProt Entry Name: ASPG_HUMAN

NCBI Description

Aspartylglucosaminidase is involved in the catabolism of N-linked oligosaccharides of glycoproteins. It cleaves asparagine from N-acetylglucosamines as one of the final steps in the lysosomal breakdown of glycoproteins. The lysosomal storage disease aspartylglycosaminuria is caused by a deficiency in the AGA enzyme. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Jan 2010]

Uniprot Description

AGA: a catabolic enzyme, aspartylglucosaminidase is involved in the catabolism of N-linked oligosaccharides of glycoproteins. It cleaves asparagine from N-acetylglucosamines as one of the final steps in the lysosomal breakdown of glycoproteins. The lysosomal storage disease aspartylglycosaminuria is caused by a deficiency in the AGA enzyme.

Protein type: Glycan Metabolism - other glycan degradation; EC 3.5.1.26; Hydrolase

Chromosomal Location of Human Ortholog: 4q34.3

Cellular Component: lysosome; endoplasmic reticulum

Molecular Function: peptidase activity; protein self-association; N4-(beta-N-acetylglucosaminyl)-L-asparaginase activity

Biological Process: protein maturation; protein deglycosylation; proteolysis

Disease: Aspartylglucosaminuria