Human Cyr61 ELISA Kit | Cyr61 elisa kit

Human Cyr61 (Cysteine Rich Protein, Angiogenic Inducer 61) ELISA Kit

Cat. Num. AAA2510311

• Gene Names: CYR61; CCN1; GIG1; IGFBP10
• Reactivity: Human
• Synonyms: Cyr61; Human Cyr61 (Cysteine Rich Protein; Angiogenic Inducer 61) ELISA Kit; Cyr61 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human Cyr61. No significant cross-reactivity or interference between Human Cyr61 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human Cyr61 and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 381

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Cyr61 elisa kit

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Cyr61 in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Cyr61. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human Cyr61 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Cyr61, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human Cyr61. You can calculate the concentration of Human Cyr61 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 49168524
• NCBI GeneID: 3491
• UniProt Accession #: Q6FI18; O00622
• Molecular Weight: 42,027 Da
• NCBI Official Full Name: CYR61
• NCBI Official Synonym Full Names: cysteine-rich, angiogenic inducer, 61
• NCBI Official Symbol: CYR61
• NCBI Official Synonym Symbols: CCN1; GIG1; IGFBP10
• NCBI Protein Information: protein CYR61; CCN family member 1; IBP-10; IGF-binding protein 10; IGFBP-10; cysteine-rich heparin-binding protein 61; cysteine-rich, anigogenic inducer, 61; insulin-like growth factor-binding protein 10
• UniProt Protein Name: CYR61 protein
• Protein Family: Protein
• UniProt Gene Name: CYR61
• UniProt Entry Name: Q6FI18_HUMAN

NCBI Description

The secreted protein encoded by this gene is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation. [provided by RefSeq, Sep 2011]

Uniprot Description

CYR61: Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up- regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. Belongs to the CCN family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p22.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix

Molecular Function: heparin binding; integrin binding; insulin-like growth factor binding; extracellular matrix binding

Biological Process: extracellular matrix organization and biogenesis; anatomical structure morphogenesis; positive regulation of caspase activity; chemotaxis; intussusceptive angiogenesis; osteoblast differentiation; positive regulation of osteoblast differentiation; cell proliferation; cell-cell signaling; positive regulation of protein kinase activity; positive regulation of osteoblast proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of cell growth; positive regulation of protein amino acid phosphorylation; cell adhesion; positive regulation of BMP signaling pathway; negative regulation of apoptosis; positive regulation of cell migration