Typical Testing Data/Standard Curve (for reference only)
Mouse Gal ELISA Kit | alpha-Gal elisa kit
Mouse Gal (alpha-galactoyl) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to alpha-Gal. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for alpha-Gal and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain alpha-Gal, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of alpha-Gal. You can calculate the concentration of alpha-Gal in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
Galanin is small neuropeptide that functions as a cellular messenger within the central and peripheral nervous systems, modulating diverse physiologic functions (Mechenthaler, 2008 [PubMed 18500643]).[supplied by OMIM, Jan 2009]
Uniprot Description
Galanin: Contracts smooth muscle of the gastrointestinal and genitourinary tract, regulates growth hormone release, modulates insulin release, and may be involved in the control of adrenal secretion. Belongs to the galanin family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 11q13.3
Cellular Component: Golgi apparatus; extracellular space; cell soma; extracellular region; secretory granule
Molecular Function: neuropeptide hormone activity; type 3 galanin receptor binding; protein binding; type 2 galanin receptor binding; type 1 galanin receptor binding
Biological Process: response to drug; nervous system development; positive regulation of cortisol secretion; positive regulation of apoptosis; positive regulation of catagen; response to insulin stimulus; negative regulation of lymphocyte proliferation; cAMP-mediated signaling; neuropeptide signaling pathway; insulin secretion; response to estrogen stimulus; regulation of glucocorticoid metabolic process; positive regulation of transcription from RNA polymerase II promoter; feeding behavior; inflammatory response
Disease: Epilepsy, Familial Temporal Lobe, 8
Research Articles on alpha-Gal
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Product Notes
The Mouse alpha-Gal gal (Catalog #AAA2508918) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2508918 ELISA Kit recognizes Mouse alpha-Gal. It is sometimes possible for the material contained within the vial of "Gal, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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