Mouse PKN1 ELISA Kit | PKN1 elisa kit

Mouse PKN1 (Protein Kinase N1) ELISA Kit

Cat. Num. AAA2506456

• Gene Names: PKN1; DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA
• Reactivity: Mouse
• Synonyms: PKN1; Mouse PKN1 (Protein Kinase N1) ELISA Kit; PKN1 elisa kit

• Reactivity: Mouse
• Specificity: This kit recognizes natural and recombinant Mouse PKN1. No significant cross-reactivity or interference between Mouse PKN1 and analogues was observed.
• Sequence Length: 603

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 1.563-100ng/mL
• Sensitivity: Min: 0.938ng/mL; Max: 100ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PKN1 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse PKN1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PKN1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PKN1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PKN1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PKN1. You can calculate the concentration of PKN1 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 63100313
• NCBI GeneID: 5585
• UniProt Accession #: Q16512; Q15143; Q504U4; Q8IUV5; Q9UD44; A8K7W5; B2R9R4; B3KVN3
• Molecular Weight: 66,060 Da
• NCBI Official Full Name: PKN1 protein
• NCBI Official Synonym Full Names: protein kinase N1
• NCBI Official Symbol: PKN1
• NCBI Official Synonym Symbols: DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA
• NCBI Protein Information: serine/threonine-protein kinase N1; protease-activated kinase 1; protein kinase C-like 1; protein kinase C-like PKN; protein kinase C-related kinase 1; protein kinase PKN-alpha; protein-kinase C-related kinase 1; serine-threonine kinase N; serine-threonine protein kinase N; serine/threonine protein kinase N
• UniProt Protein Name: Serine/threonine-protein kinase N1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PKN1
• UniProt Synonym Gene Names: PAK1; PKN; PRK1; PRKCL1; PAK-1
• UniProt Entry Name: PKN1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

PKN1: an AGC kinase of the PKN family. A PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcriptional regulation. Regulates the cytoskeletal network by phosphorylating intermediate filament proteins such as VIM and NFH, NFL and NFM, inhibiting their polymerization. Phosphorylates Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of H3T11, a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3K9me by JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, impairing their import in the nucleus. Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids. Activated by CASP3 cleavage during apoptosis. Localization to endosomes is mediated via its interaction with RHOB. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis. Three isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, AGC; EC 2.7.11.13; AGC group; PKN family

Chromosomal Location of Human Ortholog: 19p13.12

Cellular Component: cytoplasmic membrane-bound vesicle; cytoplasm; plasma membrane; midbody; nucleus; endosome; cleavage furrow

Molecular Function: protein kinase C activity; histone binding; histone deacetylase binding; Rac GTPase binding; protein kinase activity; protein serine/threonine kinase activity; protein binding; androgen receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; protein kinase C binding; GTP-Rho binding; chromatin binding; ATP binding

Biological Process: regulation of transcription from RNA polymerase II promoter; hyperosmotic response; transcription, DNA-dependent; signal transduction; protein amino acid phosphorylation; activation of JNK activity