Typical Testing Data/Standard Curve (for reference only)
Chicken APC ELISA Kit | APC elisa kit
Chicken APC (Activated Protein C) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to APC. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for APC and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain APC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of APC. You can calculate the concentration of APC in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Jul 2008]
Uniprot Description
APC: a tumor suppressor. Defects in APC are a cause of familial adenomatous polyposis (FAP). Promotes rapid degradation of CTNNB1 and participates in Wnt signaling. Activity is correlated with its phosphorylation state. Two splice-variant isoforms have been described.
Protein type: Tumor suppressor; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 5q21-q22
Cellular Component: nucleoplasm; kinetochore; centrosome; cell-cell adherens junction; tight junction; lamellipodium; cytoplasmic microtubule; cytoplasm; plasma membrane; nucleus; beta-catenin destruction complex; cytosol; lateral plasma membrane
Molecular Function: microtubule plus-end binding; protein binding; cadherin binding; gamma-catenin binding; microtubule binding; beta-catenin binding; protein kinase regulator activity; protein kinase binding
Biological Process: positive regulation of cell adhesion; apoptosis; positive regulation of apoptosis; somatic stem cell maintenance; positive regulation of microtubule polymerization; T cell differentiation in the thymus; Wnt receptor signaling pathway through beta-catenin; chromosome organization and biogenesis; cytoplasmic microtubule organization and biogenesis; anterior/posterior pattern formation; muscle maintenance; negative regulation of cell proliferation; regulation of osteoclast differentiation; cytokinesis after mitosis; hair follicle development; mitotic cell cycle spindle assembly checkpoint; positive regulation of epithelial cell differentiation; protein complex assembly; cell adhesion; cell cycle arrest; kidney development; proximal/distal pattern formation; cell structure disassembly during apoptosis; regulation of microtubule-based process; cell migration; regulation of attachment of spindle microtubules to kinetochore; negative regulation of odontogenesis; thymus development; regulation of osteoblast differentiation; negative regulation of microtubule depolymerization; negative regulation of MAPKKK cascade; axis specification; negative regulation of cyclin-dependent protein kinase activity; positive regulation of pseudopodium formation; axonogenesis; dorsal/ventral pattern formation; retina development in camera-type eye; positive regulation of cell division; positive regulation of protein catabolic process; regulation of nitrogen compound metabolic process; response to DNA damage stimulus; mitotic metaphase/anaphase transition; positive regulation of cell migration
Disease: Familial Adenomatous Polyposis 1; Gastric Cancer; Desmoid Disease, Hereditary; Colorectal Cancer; Hepatocellular Carcinoma
Research Articles on APC
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Product Notes
The Chicken APC apc (Catalog #AAA2505012) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2505012 ELISA Kit recognizes Chicken APC. It is sometimes possible for the material contained within the vial of "APC, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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