Typical Testing Data/Standard Curve (for reference only)
Mouse SYT1 ELISA Kit | SYT1 elisa kit
Mouse SYT1 (Synaptotagmin 1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SYT1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse SYT1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SYT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse SYT1. You can calculate the concentration of Mouse SYT1 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010]
Uniprot Description
SYT1: an integral membrane protein of synaptic vesicles thought to serve as a Ca(2+) sensor in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse. Binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
Protein type: Membrane protein, integral; Calcium-binding; Vesicle
Chromosomal Location of Human Ortholog: 12cen-q21
Cellular Component: presynaptic membrane; SNARE complex; synaptic vesicle; neuron projection; synaptic vesicle membrane; integral to membrane; plasma membrane; excitatory synapse; cell junction
Molecular Function: protein C-terminus binding; identical protein binding; clathrin binding; calcium-dependent phospholipid binding; transporter activity; calcium ion binding; calcium-dependent protein binding; calmodulin binding; protein binding; phosphatidylinositol-4,5-bisphosphate binding; SNARE binding; low-density lipoprotein receptor binding; phosphatidylserine binding; syntaxin-1 binding; phosphatidylinositol binding
Biological Process: regulation of exocytosis; synaptic transmission; positive regulation of calcium ion-dependent exocytosis; synaptic vesicle exocytosis; regulation of synaptic transmission, glutamatergic; vesicle docking; glutamate secretion; neurotransmitter secretion; positive regulation of vesicle fusion; pathogenesis; detection of calcium ion; protein homooligomerization; positive regulation of synaptic transmission
Research Articles on SYT1
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Product Notes
The Mouse SYT1 syt1 (Catalog #AAA2504545) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2504545 ELISA Kit recognizes Mouse SYT1. It is sometimes possible for the material contained within the vial of "SYT1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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