Human TAF ELISA Kit | TAF elisa kit

Human TAF (TATA Box Binding Protein/TBP-Associated Factors) ELISA Kit

Cat. Num. AAA2504326

• Gene Names: TAF8; 43; II; TAF; TBN; TAFII43; TAFII-43
• Reactivity: Human
• Synonyms: TAF; Human TAF (TATA Box Binding Protein/TBP-Associated Factors) ELISA Kit; TAF elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human TAF. No significant cross-reactivity or interference between Human TAF and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human TAF and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 310

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/mL
• Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TAF elisa kit

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TAF in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TAF. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human TAF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TAF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human TAF. You can calculate the concentration of Human TAF in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 104485446
• NCBI GeneID: 129685
• NCBI Accession #: NP_612639.2
• NCBI GenBank Nucleotide #: NM_138572.2
• UniProt Accession #: Q7Z7C8; Q5T0K1; Q8N4R9; Q96M52
• Molecular Weight: 37,411 Da
• NCBI Official Full Name: transcription initiation factor TFIID subunit 8
• NCBI Official Synonym Full Names: TAF8 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 43kDa
• NCBI Official Symbol: TAF8
• NCBI Official Synonym Symbols: 43; II; TAF; TBN; TAFII43; TAFII-43
• NCBI Protein Information: transcription initiation factor TFIID subunit 8; TAF(II)43; TATA box binding protein (TBP)-associated factor, RNA polymerase II, A, 45/50kDa; TBP-associated factor 43 kDa; TBP-associated factor 8; TBP-associated factor TAFII43; TBP-associated factor, RNA polymerase II, 43 kD; hTAFII43; protein taube nuss; taube nuss homolog; transcription initiation factor TFIID 43 kDa subunit
• UniProt Protein Name: Transcription initiation factor TFIID subunit 8
• Protein Family: Tafazzin
• UniProt Gene Name: TAF8
• UniProt Synonym Gene Names: TAFII43; TBN; TAFII-43; TAFII43; hTAFII43
• UniProt Entry Name: TAF8_HUMAN

NCBI Description

This gene encodes one of several TATA-binding protein (TBP)-associated factors (TAFs), which are integral subunits of the general transcription factor complex TFIID. TFIID recognizes the core promoter of many genes and nucleates the assembly of a transcription preinitiation complex containing RNA polymerase II and other initiation factors. The protein encoded by this gene contains an H4-like histone fold domain, and interacts with several subunits of TFIID including TBP and the histone-fold protein TAF10. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TBN: Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. Mediates both basal and activator-dependent transcription. Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however, does not seem to play a role in differentiation of myoblasts. Required for the integration of TAF10 in the TAF complex. May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo. Belongs to the TAF8 family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: nucleoplasm; transcription factor TFIID complex; perinuclear region of cytoplasm; nucleus

Molecular Function: protein binding; protein heterodimerization activity

Biological Process: transcription, DNA-dependent; regulation of fat cell differentiation; positive regulation of transcription, DNA-dependent; maintenance of protein localization in nucleus; cell differentiation; inner cell mass cell proliferation