Chicken CYP26A1 ELISA Kit | CYP26A1 elisa kit

Chicken CYP26A1 (Cytochrome P450 26A1) ELISA Kit

Cat. Num. AAA2504161

• Gene Names: CYP26A1; CP26; CYP26; P450RAI; P450RAI1
• Reactivity: Chicken
• Synonyms: CYP26A1; Chicken CYP26A1 (Cytochrome P450 26A1) ELISA Kit; CYP26A1 elisa kit

• Reactivity: Chicken
• Specificity: This kit recognizes natural and recombinant Chicken CYP26A1. No significant cross-reactivity or interference between Chicken CYP26A1 and analogues was observed.
• Sequence Length: 428

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CYP26A1 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken CYP26A1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CYP26A1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CYP26A1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CYP26A1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CYP26A1. You can calculate the concentration of CYP26A1 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 16933528
• NCBI GeneID: 1592
• NCBI Accession #: NP_476498.1
• NCBI GenBank Nucleotide #: NM_057157.2
• UniProt Accession #: O43174; Q5VXH9; Q5VXI0; B3KNI4
• Molecular Weight: 48,558 Da
• NCBI Official Full Name: cytochrome P450 26A1 isoform 2
• NCBI Official Synonym Full Names: cytochrome P450, family 26, subfamily A, polypeptide 1
• NCBI Official Symbol: CYP26A1
• NCBI Official Synonym Symbols: CP26; CYP26; P450RAI; P450RAI1
• NCBI Protein Information: cytochrome P450 26A1; P450, retinoic acid-inactivating, 1; cytochrome P450 retinoic acid-inactivating 1; cytochrome P450, subfamily XXVIA, polypeptide 1; cytochrome P450RAI; hP450RAI; retinoic acid 4-hydroxylase; retinoic acid-metabolizing cytochrome
• UniProt Protein Name: Cytochrome P450 26A1
• Protein Family: Cytochrome
• UniProt Gene Name: CYP26A1
• UniProt Synonym Gene Names: CYP26; P450RAI1; Cytochrome P450RAI; hP450RAI
• UniProt Entry Name: CP26A_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein acts on retinoids, including all-trans-retinoic acid (RA), with both 4-hydroxylation and 18-hydroxylation activities. This enzyme regulates the cellular level of retinoic acid which is involved in regulation of gene expression in both embryonic and adult tissues. Two alternatively spliced transcript variants of this gene, which encode the distinct isoforms, have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP26A1: Plays a key role in retinoic acid metabolism. Acts on retinoids, including all-trans-retinoic acid (RA) and its stereoisomer 9-cis-RA. Capable of both 4-hydroxylation and 18- hydroxylation. Responsible for generation of several hydroxylated forms of RA, including 4-OH-RA, 4-oxo-RA and 18-OH-RA. Belongs to the cytochrome P450 family.

Protein type: Cofactor and Vitamin Metabolism - retinol; Endoplasmic reticulum; Oxidoreductase; EC 1.14.-.-

Chromosomal Location of Human Ortholog: 10q23-q24

Cellular Component: endoplasmic reticulum membrane

Molecular Function: retinoic acid binding; iron ion binding; retinoic acid 4-hydroxylase activity; heme binding; oxygen binding

Biological Process: anterior/posterior pattern formation; retinoic acid receptor signaling pathway; negative regulation of retinoic acid receptor signaling pathway; central nervous system development; vitamin metabolic process; neural crest cell development; metabolic process; xenobiotic metabolic process