Monkey C2 ELISA Kit | C2 elisa kit

Monkey C2 (Complement Component 2) ELISA Kit

Cat. Num. AAA2502979

• Reactivity: Monkey
• Synonyms: C2; Monkey C2 (Complement Component 2) ELISA Kit; C2 elisa kit

• Reactivity: Monkey
• Specificity: This kit recognizes Monkey C2 in samples. No significant cross-reactivity or interference between Monkey C2 and analogues was observed.
• Sequence Length: 577

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.31-20ng/mL
• Sensitivity: 0.19ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Monkey C2 were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Monkey C2 were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Related Product Information for C2 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey C2 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey C2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey C2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey C2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Monkey C2. You can calculate the concentration of Monkey C2 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 15277207
• NCBI GeneID: 717
• UniProt Accession #: P06681; O19694; Q13904; B4DPF3; B4DV20; E9PFN7
• Molecular Weight: 69,444 Da
• NCBI Official Full Name: C2, partial
• UniProt Protein Name: Complement C2
• Protein Family: Complement C2
• UniProt Gene Name: C2
• UniProt Entry Name: CO2_HUMAN

NCBI Description

Component C2 is a serum glycoprotein that functions as part of the classical pathway of the complement system. Activated C1 cleaves C2 into C2a and C2b. The serine proteinase C2a then combines with complement factor 4b to create the C3 or C5 convertase. Deficiency of C2 has been reported to associated with certain autoimmune diseases and SNPs in this gene have been associated with altered susceptibility to age-related macular degeneration. This gene localizes within the class III region of the MHC on the short arm of chromosome 6. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described in publications but their full-length sequence has not been determined.[provided by RefSeq, Mar 2009]

Uniprot Description

C2: Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor 4b to generate the C3 or C5 convertase. Defects in C2 are the cause of complement component 2 deficiency (C2D). A deficiency of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus erythematosus. Skin and joint manifestations are common and renal disease is relatively rare. Patients with complement component 2 deficiency are also reported to have recurrent or invasive infections. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Protease; Secreted; EC 3.4.21.43

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular space; extracellular region

Molecular Function: metal ion binding; serine-type endopeptidase activity

Biological Process: regulation of complement activation; innate immune response; proteolysis; complement activation, classical pathway; response to nutrient; complement activation

Disease: Macular Degeneration, Age-related, 14; Complement Component 2 Deficiency