Chicken eIF3a ELISA Kit | eIF3a elisa kit

Chicken eIF3a (Eukaryotic Translation Initiation Factor 3a) ELISA Kit

Cat. Num. AAA2502748

• Gene Names: eif3s10; zgc:66194; zgc:77263; wu:fi15c03
• Reactivity: Chicken
• Synonyms: eIF3a; Chicken eIF3a (Eukaryotic Translation Initiation Factor 3a) ELISA Kit; eIF3a elisa kit

• Reactivity: Chicken
• Specificity: This kit recognizes natural and recombinant Chicken eIF3a. No significant cross-reactivity or interference between Chicken eIF3a and analogues was observed.
• Sequence Length: 1267

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 3.125-200pg/mL
• Sensitivity: Min: 1.875pg/mL; Max: 200pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for eIF3a elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken eIF3a concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to eIF3a. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for eIF3a and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain eIF3a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of eIF3a. You can calculate the concentration of eIF3a in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 45433533
• NCBI GeneID: 327515
• NCBI Accession #: NP_956114.2
• NCBI GenBank Nucleotide #: NM_199820.2
• UniProt Accession #: Q6PCR7; Q6NYB1
• Molecular Weight: 151,292 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 3 subunit A
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 3, subunit 10 (theta)
• NCBI Official Symbol: eif3s10
• NCBI Official Synonym Symbols: zgc:66194; zgc:77263; wu:fi15c03
• NCBI Protein Information: eukaryotic translation initiation factor 3 subunit A
• UniProt Protein Name: Eukaryotic translation initiation factor 3 subunit A
• Protein Family: Eukaryotic translation initiation factor
• UniProt Gene Name: eif3a
• UniProt Entry Name: EIF3A_DANRE

Uniprot Description

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.