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Immunohistochemistry (IHC) (Anti-ETS2 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:75.)

Rabbit anti-Human ETS2 Polyclonal Antibody | anti-ETS2 antibody

Anti-ETS2 Antibody (aa146-173) IHC-plus

Gene Names
ETS2; ETS2IT1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
Purity
Immunoaffinity purified
Synonyms
ETS2; Polyclonal Antibody; Anti-ETS2 Antibody (aa146-173) IHC-plus; Rabbit Polyclonal to Human ETS2; Human ETS2; c-ets2; Oncogene ETS-2; Protein C-ets-2; ETS2IT1; anti-ETS2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Human ETS2
Purity/Purification
Immunoaffinity purified
Form/Format
PBS, 0.09% sodium azide
Sequence Length
469
Applicable Applications for anti-ETS2 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Flow Cytometry (1:10 - 1:50)
IHC-P (1:75)
WB (1:1000)
Immunogen
ETS2 antibody was raised against kLH-conjugated synthetic peptide from internal region of human ETS2.
Immunogen Description
KLH-conjugated synthetic peptide from internal region of human ETS2.
Immunogen Type
Synthetic peptide
Antigen Modification
aa146-173
Target Species
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months.
For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-ETS2 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:75.)

Immunohistochemistry (IHC) (Anti-ETS2 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:75.)

Western Blot (WB)

(Western blot of ETS2 Antibody in MCF-7 cell line lysates (35 ug/lane). ETS2 (arrow) was detected using the purified antibody.)

Western Blot (WB) (Western blot of ETS2 Antibody in MCF-7 cell line lysates (35 ug/lane). ETS2 (arrow) was detected using the purified antibody.)

Flow Cytometry (FC/FACS)

(ETS2 Antibody flow cytometry of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (ETS2 Antibody flow cytometry of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-ETS2 antibody
ETS2 Antibody, c-ets2 Antibody, Oncogene ETS-2 Antibody, Protein C-ets-2 Antibody, ETS2IT1 Antibody Description: Transcription factor activating transcription. Binds specifically the DNA GGAA/T core motif (Ets-binding site or EBS) in gene promoters and stimulates transcription.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
53,001 Da
NCBI Official Full Name
protein C-ets-2 isoform 1
NCBI Official Synonym Full Names
v-ets avian erythroblastosis virus E26 oncogene homolog 2
NCBI Official Symbol
ETS2
NCBI Official Synonym Symbols
ETS2IT1
NCBI Protein Information
protein C-ets-2
UniProt Protein Name
Protein C-ets-2
Protein Family
UniProt Gene Name
ETS2
UniProt Entry Name
ETS2_HUMAN

NCBI Description

This gene encodes a transcription factor which regulates genes involved in development and apoptosis. The encoded protein is also a protooncogene and shown to be involved in regulation of telomerase. A pseudogene of this gene is located on the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

Ets-2: a proto-oncogenic transcription factor homologous to the v-ets erythroblastosis virus E26 oncogene. A target of the Akt/JNK signaling pathway in macrophages. A PKC pathway activates ETS2, up-regulating GM-CSF in non-small lung carcinoma cells.

Protein type: Oncoprotein; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 21q22.2

Cellular Component: cytoplasm; nucleoplasm; nucleus; plasma membrane

Molecular Function: DNA binding; glucocorticoid receptor binding; protein binding; transcription factor activity

Biological Process: cell differentiation; ectodermal cell fate commitment; mesoderm development; negative regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; skeletal development; transcription, DNA-dependent

Research Articles on ETS2

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Product Notes

The ETS2 ets2 (Catalog #AAA249904) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ETS2 Antibody (aa146-173) IHC-plus reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ETS2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS). Flow Cytometry (1:10 - 1:50) IHC-P (1:75) WB (1:1000). Researchers should empirically determine the suitability of the ETS2 ets2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ETS2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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