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Immunohistochemistry (IHC)
(Anti-Myelin Basic Protein / MBP antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)
Mouse anti-Human Myelin Basic Protein / MBP Monoclonal Antibody | anti-MBP antibody
Mouse Monoclonal [clone 2H9] (IgG1) to Human Myelin Basic Protein / MBP
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Western Blot, Flow Cytometry, Functional Assay, ELISA
Purity
Ascites
Synonyms
Myelin Basic Protein / MBP; Monoclonal Antibody; Mouse Monoclonal [clone 2H9] (IgG1) to Human Myelin Basic Protein / MBP; Anti-Myelin Basic Protein / MBP Antibody (clone 2H9) IHC-plus; MBP; Myelin A1 protein; Myelin basic protein; Human Myelin Basic Protein; anti-MBP antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2H9
Specificity
Human Myelin Basic Protein / MBP
Purity/Purification
Ascites
Form/Format
Ascites, 0.03% sodium azide
Sequence Length
186
Applicable Applications for anti-MBP antibody
Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes
ELISA (1:10000), Flo (1:200 - 1:400), ICC (1:200 - 1:1000), IF (1:1000), IHC-P (1:100), WB (1:500 - 1:2000)
Target Species
Human
Immunogen Description
Purified recombinant fragment of human MBP expressed in E Coli.
Immunogen Type
Recombinant protein
Immunogen
Myelin Basic Protein / MBP antibody was raised against purified recombinant fragment of human MBP expressed in E Coli.
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.
Immunohistochemistry (IHC)
(Anti-Myelin Basic Protein / MBP antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)
Immunohistochemistry (IHC)
(Anti-Myelin Basic Protein / MBP antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)
Immunohistochemistry (IHC)
(Anti-Myelin Basic Protein / MBP antibody IHC staining of human skeletal muscle, nerve. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Immunohistochemistry (IHC)
(Anti-Myelin Basic Protein / MBP antibody IHC staining of human skeletal muscle, nerve. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Immunofluorescence (IF)
(Immunofluorescence of MSCS cells using MBP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
Immunofluorescence (IF)
(Immunofluorescence of MSCS cells using MBP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
Flow Cytometry (FC/FACS)
(Flow cytometry of HepG2 cells using MBP mouse mAb (green) and negative control (red).)
Flow Cytometry (FC/FACS)
(Flow cytometry of HepG2 cells using MBP mouse mAb (green) and negative control (red).)
Western Blot (WB)
(Western blot of MBP mAb against HEK293 (1) and MBP-hIgGFc transfected HEK293 (2) cell lysate.)
Western Blot (WB)
(Western blot of MBP mAb against HEK293 (1) and MBP-hIgGFc transfected HEK293 (2) cell lysate.)
Western Blot (WB)
(Western blot of MBP mAb against human MBP (AA: 1-197) recombinant protein. (Expected MW is 70 kDa))
Western Blot (WB)
(Western blot of MBP mAb against human MBP (AA: 1-197) recombinant protein. (Expected MW is 70 kDa))
ELISA (EIA)
(Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);)
ELISA (EIA)
(Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);)
Related Product Information for anti-MBP antibody
The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17,343 Da
NCBI Official Full Name
myelin basic protein isoform 2
NCBI Official Synonym Full Names
myelin basic protein
NCBI Official Symbol
MBP
NCBI Protein Information
Golli-MBP; myelin basic protein; myelin A1 protein; myelin membrane encephalitogenic protein
UniProt Protein Name
Myelin basic protein
Protein Family
UniProt Gene Name
MBP
UniProt Synonym Gene Names
MBP
UniProt Entry Name
MBP_HUMAN
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Product Notes
The MBP mbp (Catalog #AAA249055) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 2H9] (IgG1) to Human Myelin Basic Protein / MBP reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Myelin Basic Protein / MBP can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA). ELISA (1:10000), Flo (1:200 - 1:400), ICC (1:200 - 1:1000), IF (1:1000), IHC-P (1:100), WB (1:500 - 1:2000). Researchers should empirically determine the suitability of the MBP mbp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myelin Basic Protein / MBP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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