Rabbit FFAR2 / GPR43 Polyclonal Antibody | anti-FFAR2 antibody

Rabbit Polyclonal to Human FFAR2 / GPR43

Cat. Num. AAA248885

• Gene Names: FFAR2; FFA2R; GPR43
• Reactivity: Human, Monkey, Mouse, Rat, Pig; Predicted Reactivity: Bat, Bovine, Goat, Horse
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: FFAR2 / GPR43; Polyclonal Antibody; Rabbit Polyclonal to Human FFAR2 / GPR43; Anti-FFAR2 / GPR43 Antibody (Cytoplasmic Domain) IHC-plus; FFAR2; FFA2R; Free fatty acid receptor 2; G protein-coupled receptor 43; G-protein coupled receptor 43; LSSIG; GPR43; Gpcr43; Human FFAR2; anti-FFAR2 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat, Pig; Predicted Reactivity: Bat, Bovine, Goat, Horse
• Clonality: Polyclonal
• Specificity: Human GPR43. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except FFAR1 (58%).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, less than 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 330

• Applicable Applications for anti-FFAR2 antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (5-10 ug/ml); Optimal dilution to be determined by researcher
• Antigen Type: Synthetic peptide
• Immunogen Species: FFAR2/GPR43 antibody was raised against Human.
• Immunogen: FFAR2 / GPR43 antibody was raised against synthetic 19 amino acid peptide from 2nd cytoplasmic domain of human GPR43. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat, Elephant, Pig (100%); Bovine, Bat, Goat, Horse, Opossum (95%); Dog, Panda (89%); Rabbit (84%).
• Epitope: Cytoplasmic Domain
• Important Note: During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Upon arrival: Aliquot and store undiluted at -20 degree C or below for up to 1 year. Can be stored undulited at 4 degree C for up to one month. Avoid repeated freeze thaw cycles.

Related Product Information for anti-FFAR2 antibody

GPR43 is classified as a Carboxylic Acid GPCR. The location of this receptor in the immune system is suggestive of potential roles in immune cell function and hematopoiesis.

Product Categories/Family for anti-FFAR2 antibody

Family: GPCR; Subfamily: Carboxylic Acid

NCBI and Uniprot Product Information

• NCBI GI #: 4885333
• NCBI GeneID: 2867
• NCBI Accession #: NP_005297.1
• NCBI GenBank Nucleotide #: NM_005306.2
• UniProt Accession #: O15552; Q4VAZ3; Q4VAZ5; Q4VBL5; B0M0J9
• NCBI Official Full Name: free fatty acid receptor 2
• NCBI Official Synonym Full Names: free fatty acid receptor 2
• NCBI Official Symbol: FFAR2
• NCBI Official Synonym Symbols: FFA2R; GPR43
• NCBI Protein Information: free fatty acid receptor 2; G protein-coupled receptor 43; G-protein coupled receptor 43; free fatty acid activated receptor 2
• UniProt Protein Name: Free fatty acid receptor 2
• Protein Family: Free fatty acid receptor
• UniProt Gene Name: FFAR2
• UniProt Synonym Gene Names: FFA2; GPCR43; GPR43
• UniProt Entry Name: FFAR2_HUMAN

NCBI Description

This gene encodes a member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for short chain free fatty acids and may be involved in the inflammatory response and in regulating lipid plasma levels. [provided by RefSeq, Apr 2009]

Uniprot Description

FFAR2: Receptor for short chain fatty acids through a G(i)- protein-mediated inhibition of adenylyl cyclase and elevation of intracellular calcium. The rank order of potency for agonists of this receptor is acetate= propionate = butyrate > pentanoate = formate. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cell projection; integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding; lipid binding

Biological Process: fat cell differentiation; G-protein coupled receptor protein signaling pathway; sequestering of lipid; mucosal immune response; regulation of acute inflammatory response; positive regulation of cytokine production during immune response; cell surface pattern recognition receptor signaling pathway; positive regulation of acute inflammatory response to non-antigenic stimulus; glucose homeostasis; leukocyte chemotaxis during inflammatory response; positive regulation of chemokine production