Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Immunohistochemistry (IHC) (Anti-TRAM1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Rabbit anti-Human TRAM1 Polyclonal Antibody | anti-TRAM1 antibody

Rabbit Polyclonal to Human TRAM1

Gene Names
TRAM1; TRAM; PNAS8; TRAMP
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Immunoaffinity Purified
Synonyms
TRAM1; Polyclonal Antibody; Rabbit Polyclonal to Human TRAM1; Anti-TRAM1 Antibody (aa310-374) IHC-plus; PNAS8; TRAM; TRAMP; Human TRAM1; anti-TRAM1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Human TRAM1
Purity/Purification
Immunoaffinity Purified
Form/Format
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Concentration
1 mg/ml (varies by lot)
Sequence Length
374
Applicable Applications for anti-TRAM1 antibody
Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes
ICC (1:100 - 1:1000), IHC-P (1:100), WB (1:500 - 1:3000)
Usage: IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Target Species
Human
Immunogen Description
Synthetic peptide corresponding to a region within amino acids 310 and 374 of TRAM1 (SwissProt Q15629).
Immunogen Type
Synthetic peptide
Immunogen
TRAM1 antibody was raised against synthetic peptide corresponding to a region within amino acids 310 and 374 of TRAM1 (SwissProt Q15629).
Antigen Modification
aa310-374
Preparation and Storage
Keep as concentrated solution. Aliquot and store at -20 degree C or below. Avoid multiple freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-TRAM1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC) (Anti-TRAM1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-TRAM1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Immunohistochemistry (IHC) (Anti-TRAM1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Immunocytochemistry (ICC)

(Immunofluorescence of methanol-fixed HeLa using TRAM1 antibody at 1:500 dilution.)

Immunocytochemistry (ICC) (Immunofluorescence of methanol-fixed HeLa using TRAM1 antibody at 1:500 dilution.)

Western Blot (WB)

(Sample (30 ug of whole cell lysate). A: H1299. 10% SDS PAGE. TRAM1 antibody diluted at 1:1000.)

Western Blot (WB) (Sample (30 ug of whole cell lysate). A: H1299. 10% SDS PAGE. TRAM1 antibody diluted at 1:1000.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39,683 Da
NCBI Official Full Name
translocating chain-associated membrane protein 1
NCBI Official Synonym Full Names
translocation associated membrane protein 1
NCBI Official Symbol
TRAM1
NCBI Official Synonym Symbols
TRAM; PNAS8; TRAMP
NCBI Protein Information
translocating chain-associated membrane protein 1; translocation-associating membrane protein 1; translocating chain-associating membrane protein
UniProt Protein Name
Translocating chain-associated membrane protein 1
UniProt Gene Name
TRAM1
UniProt Synonym Gene Names
TRAM
UniProt Entry Name
TRAM1_HUMAN

NCBI Description

This gene encodes a multi-pass membrane protein that is part of the mammalian endoplasmic reticulum. The encoded protein influences glycosylation and facilitates the translocation of secretory proteins across the endoplasmic reticulum membrane by regulating which domains of the nascent polypeptide chain are visible to the cytosol during a translocational pause. [provided by RefSeq, Oct 2009]

Uniprot Description

TRAM1: Stimulatory or required for the translocation of secretory proteins across the ER membrane. Belongs to the TRAM family.

Protein type: Membrane protein, multi-pass; Receptor, misc.; Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 8q13.3

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Molecular Function: protein binding; receptor activity

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translation; viral reproduction; gene expression; cotranslational protein targeting to membrane

Research Articles on TRAM1

Similar Products

Product Notes

The TRAM1 tram1 (Catalog #AAA248608) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human TRAM1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRAM1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB). ICC (1:100 - 1:1000), IHC-P (1:100), WB (1:500 - 1:3000) Usage: IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0). Researchers should empirically determine the suitability of the TRAM1 tram1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRAM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.