Goat ETFB Polyclonal Antibody | anti-ETFB antibody

Goat Polyclonal to Human ETFB

Cat. Num. AAA248471

• Gene Names: ETFB; MADD; FP585
• Reactivity: Gibbon, Gorilla, Human, Orangutan; Predicted Reactivity: Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: ETFB; Polyclonal Antibody; Goat Polyclonal to Human ETFB; Anti-ETFB Antibody (aa152-165) IHC-plus; FP585; Beta-ETF; Human ETFB; anti-ETFB antibody

• Host: Goat
• Reactivity: Gibbon, Gorilla, Human, Orangutan; Predicted Reactivity: Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human ETFB. This antibody is expected to recognize both reported isoforms (NP_001976.1; NP_001014763.1). Amino acid numbering in the name above refers to NP_001976.1).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 255

• Applicable Applications for anti-ETFB antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:8000), IHC-P (3.75 ug/ml), WB (1 - 3 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 27kD band observed in Human, Mouse and Rat Liver lysates (calculated MW of 27.8kD according to NP_001976.1). Recommended concentration: 1-3 ug/ml.
• Target Species: Human
• Immunogen Description: Synthetic peptide C-QVTLEGDKLKVERE from an internal region of human ETFB (NP_001976.1; NP_001014763.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Elephant, Panda (100%).
• Immunogen Type: Synthetic peptide
• Immunogen: ETFB antibody was raised against synthetic peptide C-QVTLEGDKLKVERE from an internal region of human ETFB (NP_001976.1; NP_001014763.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Elephant, Panda (100%); Marmoset, Mouse, Rat, Dog, Bat, Bovine, Horse, Rabbit, Pig (93%); Hamster (86%).
• Antigen Modification: aa152-165
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-ETFB antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Western Blot (WB)

(ETFB antibody (0.1 ug/ml) staining of Human Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-ETFB antibody

Electron-transfer-flavoprotein, beta polypeptide shuttles electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. ETFB deficiencies have been implicated in type II glutaricaciduria.

NCBI and Uniprot Product Information

• NCBI GI #: 4503609
• NCBI GeneID: 2109
• NCBI Accession #: NP_001976.1
• NCBI GenBank Nucleotide #: NM_001985.2
• UniProt Accession #: P38117; Q6IBH7; Q71RF6; Q9Y3S7; A8K766; B3KNY2
• Molecular Weight: 37,434 Da
• NCBI Official Full Name: electron transfer flavoprotein subunit beta isoform 1
• NCBI Official Synonym Full Names: electron-transfer-flavoprotein, beta polypeptide
• NCBI Official Symbol: ETFB
• NCBI Official Synonym Symbols: MADD; FP585
• NCBI Protein Information: electron transfer flavoprotein subunit beta; beta-ETF; electron transfer flavoprotein beta subunit; electron transfer flavoprotein beta-subunit; electron transfer flavoprotein, beta polypeptide; electron-transferring-flavoprotein, beta polypeptide
• UniProt Protein Name: Electron transfer flavoprotein subunit beta
• Protein Family: Electron transfer flavoprotein
• UniProt Gene Name: ETFB
• UniProt Synonym Gene Names: Beta-ETF
• UniProt Entry Name: ETFB_HUMAN

NCBI Description

This gene encodes electron-transfer-flavoprotein, beta polypeptide, which shuttles electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. The gene deficiencies have been implicated in type II glutaricaciduria. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ETFB: The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl- CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Defects in ETFB are the cause of glutaric aciduria type 2B (GA2B). GA2B is an autosomal recessively inherited disorder of fatty acid, amino acid, and choline metabolism. It is characterized by multiple acyl-CoA dehydrogenase deficiencies resulting in large excretion not only of glutaric acid, but also of lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Belongs to the ETF beta-subunit/FixA family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; Mitochondrial

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: mitochondrion; intracellular membrane-bound organelle; mitochondrial matrix

Molecular Function: electron carrier activity

Biological Process: cellular metabolic process

Disease: Multiple Acyl-coa Dehydrogenase Deficiency