Rabbit anti-Human RPS16 / S16 Polyclonal Antibody | anti-RPS16 antibody

Rabbit Polyclonal (IgG) to Human RPS16 / S16

Cat. Num. AAA248403

• Gene Names: RPS16; S16
• Reactivity: Human
• Applications: Immunohistochemistry, Immunohistochemistry, Western Blot, ELISA
• Purity: Caprylic acid and ammonium sulfate precipitation
• Synonyms: RPS16 / S16; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human RPS16 / S16; Anti-RPS16 / S16 Antibody IHC-plus; RPS16; 40S ribosomal protein S16; S16; Ribosomal protein S16; Human RPS16; anti-RPS16 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human RPS16/S16
• Purity/Purification: Caprylic acid and ammonium sulfate precipitation
• Form/Format: PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 146

• Applicable Applications for anti-RPS16 antibody: Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: IHC; IHC-Paraffin (10 ug/ml); Western Blot (2 ug/ml); ELISA; (optimal dilution to be determined by the researcher)
• Immunogen Species: RPS16/Ribosomal Protein S16 antibody was raised against Human
• Antigen Type: Recombinant protein
• Immunogen: RPS16/Ribosomal Protein S16 antibody was raised against recombinant human 40S ribosomal protein S16 protein aa 2-142.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

NCBI and Uniprot Product Information

• NCBI GI #: 4506691
• NCBI GeneID: 6217
• NCBI Accession #: NP_001011.1
• NCBI GenBank Nucleotide #: NM_001020.4
• UniProt Accession #: P62249; P17008; B2RDD5
• NCBI Official Full Name: 40S ribosomal protein S16
• NCBI Official Synonym Full Names: ribosomal protein S16
• NCBI Official Symbol: RPS16
• NCBI Official Synonym Symbols: S16
• NCBI Protein Information: 40S ribosomal protein S16
• UniProt Protein Name: 40S ribosomal protein S16
• Protein Family: 40S ribosomal protein
• UniProt Gene Name: RPS16
• UniProt Entry Name: RS16_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S9P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPS16: a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S9P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Protein type: Ribosomal; Translation

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: small ribosomal subunit; focal adhesion; membrane; cytosol

Molecular Function: protein binding; structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; translation; viral reproduction; viral infectious cycle; translational termination; ribosomal small subunit biogenesis and assembly; cellular protein metabolic process; translational elongation; translational initiation; mRNA catabolic process, nonsense-mediated decay; gene expression; viral transcription; rRNA processing