Rabbit TRIM33 / TIF1-Gamma Polyclonal Antibody | anti-TRIM33 antibody

Rabbit Polyclonal (IgG) to Human TRIM33 / TIF1-Gamma

Cat. Num. AAA248171

• Gene Names: TRIM33; ECTO; PTC7; RFG7; TF1G; TIF1G; TIFGAMMA; TIF1GAMMA
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: TRIM33 / TIF1-Gamma; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human TRIM33 / TIF1-Gamma; Anti-TRIM33 / TIF1-Gamma Antibody (Internal) IHC-plus; TRIM33; Ectodermin homolog; ECTO; RET-FUSED GENE 7; RFG7; TF1G; TIF1GAMMA; TIFGAMMA; TIF1-gamma; PTC7; RET-fused gene 7 protein; TIF1G; Tripartite motif-containing 33; KIAA1113; Protein Rfg7; Tripartite motif containing 33; Human TRIM33; TIF1-Gamma; anti-TRIM33 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TRIM33 antibody is human, mouse and rat reactive. At least two isoforms of TRIM33 are known to exist.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1127

• Applicable Applications for anti-TRIM33 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (20 ug/ml), IHC-P (10 ug/ml), WB (1 - 2 ug/ml)
• Target: TRIM33/TIF-Gamma
• Epitope: Internal
• Immunogen Description: 18 amino acid peptide near the center of human TRIM33.
• Immunogen: TRIM33 / TIF1-Gamma antibody was raised against 18 amino acid peptide near the center of human TRIM33.
• Antigen Modification: Internal
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-TRIM33 / TIF1-Gamma antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of TRIM33 in human liver tissue with TRIM33 antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot analysis of TRIM33 in human liver tissue lysate with TRIM33 antibody at (A) 1 and (B) 2 ug/ml.)

Related Product Information for anti-TRIM33 antibody

TIF1gamma is a member of the Transcriptional Intermediary Factor 1 (TIF1) subfamily. The structure of TIF1beta is similar to TIF1alpha and TIF1beta, containing a RING finger, B box, Coiled coil, PHD/TTC, and bromodomain. TIF1gamma does not interact with nuclear receptors, does not interfere with retinoic acid response in transfected mammalian cells, and does not interact with either the KRAB domain of KOX1 or the HP1 proteins.

Product Categories/Family for anti-TRIM33 antibody

Family: Tripartite Motif; Subfamily: TIF1

NCBI and Uniprot Product Information

• NCBI GI #: 74027249
• NCBI GeneID: 51592
• NCBI Accession #: NP_056990.3
• NCBI GenBank Nucleotide #: NM_015906.3
• UniProt Accession #: Q9UPN9; O95855; Q5TG72; Q5TG73; Q5TG74; Q9C017; Q9UJ79
• Molecular Weight: 120,541 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase TRIM33 isoform alpha
• NCBI Official Synonym Full Names: tripartite motif containing 33
• NCBI Official Symbol: TRIM33
• NCBI Official Synonym Symbols: ECTO; PTC7; RFG7; TF1G; TIF1G; TIFGAMMA; TIF1GAMMA
• NCBI Protein Information: E3 ubiquitin-protein ligase TRIM33; TIF1-gamma; protein Rfg7; ectodermin homolog; RET-fused gene 7 protein; tripartite motif-containing 33; transcriptional intermediary factor 1 gamma
• UniProt Protein Name: E3 ubiquitin-protein ligase TRIM33
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: TRIM33
• UniProt Synonym Gene Names: KIAA1113; RFG7; TIF1G; Protein Rfg7; TIF1-gamma
• UniProt Entry Name: TRI33_HUMAN

NCBI Description

The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TIF1G: an atypical protein kinase that functions as a transcriptional repressor. Translocation with RET generates the TRIM33/RET (PTC7) oncogene. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Two splice variant isoforms have been described.

Protein type: Kinase, protein; EC 6.3.2.19; EC 6.3.2.-; Protein kinase, atypical; Ubiquitin ligase; Ubiquitin conjugating system; Ligase; Transcription, coactivator/corepressor; ATYPICAL group; TIF1 family

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: transcription initiation from RNA polymerase II promoter; regulation of transforming growth factor beta receptor signaling pathway; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; protein ubiquitination; gene expression; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of BMP signaling pathway

Disease: Thyroid Carcinoma, Papillary