Goat CYP2E1 Polyclonal Antibody | anti-CYP2E1 antibody

Goat Polyclonal to Human CYP2E1

Cat. Num. AAA247150

• Gene Names: CYP2E1; CPE1; CYP2E; P450-J; P450C2E
• Reactivity: Gibbon, Gorilla, Human, Orangutan
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: CYP2E1; Polyclonal Antibody; Goat Polyclonal to Human CYP2E1; Anti-CYP2E1 Antibody (Internal) IHC-plus; 4-nitrophenol 2-hydroxylase; CPE1; Cytochrome p450 IIe1; Cytochrome P450-J; p450C2E; p450-J; CYP2E; CYPIIE1; Cytochrome P450 2E1; Human CYP2E1; anti-CYP2E1 antibody

• Host: Goat
• Reactivity: Gibbon, Gorilla, Human, Orangutan
• Clonality: Polyclonal
• Specificity: Human CYP2E1.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 493

• Applicable Applications for anti-CYP2E1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:32000), IHC-P (3.75 ug/ml), WB (0.05 - 0.2 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 50kD band observed in Human Liver lysates (calculated MW of 56.8kD according to NP_000764.1). Recommended concentration: 0.05-0.2 ug/ml.
• Target Species: Human
• Immunogen Description: Synthetic peptide C-RKVIKNVAEVK from an internal region of human CYP2E1 (NP_000764.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon (100%); Monkey, Marmoset, Cat, Rabbit, Trichina worm (82%).
• Immunogen Type: Synthetic peptide
• Immunogen: CYP2E1 antibody was raised against synthetic peptide C-RKVIKNVAEVK from an internal region of human CYP2E1 (NP_000764.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon (100%); Monkey, Marmoset, Cat, Rabbit, Trichina worm (82%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-CYP2E1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.)

Western Blot (WB)

(CYP2E1 antibody (0.05 ug/ml) staining of Human Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Product Categories/Family for anti-CYP2E1 antibody

Family: Cytochrome P450

NCBI and Uniprot Product Information

• NCBI GI #: 10834998
• NCBI GeneID: 1571
• NCBI Accession #: NP_000764.1
• NCBI GenBank Nucleotide #: NM_000773.3
• UniProt Accession #: P05181; Q5VZD5; Q6NWT9; Q9UK47
• Molecular Weight: 56,849 Da
• NCBI Official Full Name: cytochrome P450 2E1
• NCBI Official Synonym Full Names: cytochrome P450, family 2, subfamily E, polypeptide 1
• NCBI Official Symbol: CYP2E1
• NCBI Official Synonym Symbols: CPE1; CYP2E; P450-J; P450C2E
• NCBI Protein Information: cytochrome P450 2E1; CYPIIE1; cytochrome P450-J; microsomal monooxygenase; xenobiotic monooxygenase; 4-nitrophenol 2-hydroxylase; flavoprotein-linked monooxygenase; cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1
• UniProt Protein Name: Cytochrome P450 2E1
• Protein Family: Cytochrome
• UniProt Gene Name: CYP2E1
• UniProt Synonym Gene Names: CYP2E
• UniProt Entry Name: CP2E1_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP2E1: Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. Belongs to the cytochrome P450 family.

Protein type: Lipid Metabolism - arachidonic acid; EC 1.14.13.n7; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Endoplasmic reticulum; Xenobiotic Metabolism - metabolism by cytochrome P450; Lipid Metabolism - linoleic acid; Mitochondrial

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: Golgi membrane; intrinsic to endoplasmic reticulum membrane; endoplasmic reticulum membrane; intracellular membrane-bound organelle; mitochondrion; cytoplasm

Molecular Function: oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen; enzyme binding; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; heme binding; arachidonic acid epoxygenase activity; steroid hydroxylase activity; oxidoreductase activity; oxygen binding; monooxygenase activity

Biological Process: steroid metabolic process; triacylglycerol metabolic process; response to ethanol; response to ozone; xenobiotic metabolic process; exogenous drug catabolic process; monoterpenoid metabolic process; epoxygenase P450 pathway; heterocycle metabolic process; drug metabolic process; response to organic nitrogen