Mouse anti-Human Vitamin D Receptor / VDR Monoclonal Antibody | anti-VDR antibody

Mouse Monoclonal [clone 2F4] (IgG2a,k) to Human Vitamin D Receptor / VDR

Cat. Num. AAA246023

• Gene Names: VDR; NR1I1; PPP1R163
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Protein A Purified
• Synonyms: Vitamin D Receptor / VDR; Monoclonal Antibody; Mouse Monoclonal [clone 2F4] (IgG2a; k) to Human Vitamin D Receptor / VDR; Anti-Vitamin D Receptor / VDR Antibody (clone 2F4) IHC-plus; VDR; NR1I1; Vitamin D3 receptor; Vitamin D receptor; Human Vitamin D Receptor; anti-VDR antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 2F4
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.4. Sourced in Ascites.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 427

• Applicable Applications for anti-VDR antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml); Usage: Sandwich ELISA (Recombinant protein).
• Target Species: Human
• Immunogen Description: VDR (AAH60832, 1 a.a. ~ 428 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Type: Recombinant protein
• Immunogen: Vitamin D Receptor / VDR antibody was raised against vDR (AAH60832, 1 a.a. ~ 428 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-VDR antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

ELISA (EIA)

(Detection limit for recombinant GST tagged VDR is approximately 1 ng/ml as a capture antibody.)

Related Product Information for anti-VDR antibody

The vitamin D receptor, a NR1 Thyroid Hormone-Like Receptor, is activated by calcitriol, the active hormonal form of vitamin D. The activated receptor binds DNA at a specific vitamin D-responsive element and promotes transcription of vitamin D target genes such as osteocalcin, the most abundant noncollagenous protein in bone. The vitamin D receptor affects growth, bone formation, and female reproduction.

Product Categories/Family for anti-VDR antibody

Family: NHR; Subfamily: NR1 Thyroid hormone-like

NCBI and Uniprot Product Information

• NCBI GI #: 4507883
• NCBI GeneID: 7421
• NCBI Accession #: NP_000367.1
• NCBI GenBank Nucleotide #: NM_000376.2
• UniProt Accession #: P11473; Q5PSV3; B2R5Q1; G3V1V9
• Molecular Weight: 53,883 Da
• NCBI Official Full Name: vitamin D3 receptor isoform VDRA
• NCBI Official Synonym Full Names: vitamin D (1,25- dihydroxyvitamin D3) receptor
• NCBI Official Symbol: VDR
• NCBI Official Synonym Symbols: NR1I1; PPP1R163
• NCBI Protein Information: vitamin D3 receptor; 1,25-dihydroxyvitamin D3 receptor; vitamin D nuclear receptor variant 1; nuclear receptor subfamily 1 group I member 1; protein phosphatase 1, regulatory subunit 163
• UniProt Protein Name: Vitamin D3 receptor
• Protein Family: Vitamin D3 receptor
• UniProt Gene Name: VDR
• UniProt Synonym Gene Names: NR1I1; VDR
• UniProt Entry Name: VDR_HUMAN

NCBI Description

This gene encodes the nuclear hormone receptor for vitamin D3. This receptor also functions as a receptor for the secondary bile acid lithocholic acid. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternative splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq, Feb 2011]

Uniprot Description

VDR: Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis. Defects in VDR are the cause of rickets vitamin D- dependent type 2A (VDDR2A). A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets. Belongs to the nuclear hormone receptor family. NR1 subfamily.

Protein type: Transcription factor; DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 12q13.11

Cellular Component: nucleoplasm; nucleus; receptor complex

Molecular Function: retinoid X receptor binding; protein binding; DNA binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity; vitamin D3 receptor activity; transcription factor activity

Biological Process: lactation; transcription initiation from RNA polymerase II promoter; cell morphogenesis; positive regulation of apoptosis involved in mammary gland involution; negative regulation of transcription from RNA polymerase II promoter; decidualization; signal transduction; intestinal absorption; cellular calcium ion homeostasis; negative regulation of cell proliferation; calcium ion transport; steroid hormone mediated signaling; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; skeletal development; positive regulation of keratinocyte differentiation

Disease: Vitamin D-dependent Rickets, Type 2a; Osteoporosis