Mouse anti-Human TROVE2 Monoclonal Antibody | anti-TROVE2 antibody

Mouse Monoclonal [clone 2B4] (IgG2a,k) to Human TROVE2

Cat. Num. AAA245859

• Gene Names: TROVE2; RO60; SSA2; RORNP
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Purified from ascites by Protein A
• Synonyms: TROVE2; Monoclonal Antibody; Mouse Monoclonal [clone 2B4] (IgG2a; k) to Human TROVE2; 60 kDa ribonucleoprotein Ro; 60 kDa Ro protein; Sjoegren syndrome antigen A2; TROVE domain family member 2; Ro 60 kDa autoantigen; RORNP; SSA2; R060; SS-A; TROVE domain family; member 2; anti-TROVE2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 2B4
• Specificity: Human TROVE2
• Purity/Purification: Purified from ascites by Protein A
• Form/Format: PBS, pH 7.4.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 538

• Applicable Applications for anti-TROVE2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: IHC-P (1:100); WB (1:500 - 1:1000); (Optimal dilution to be determined by the researcher)
• Target: TROVE2
• Immunogen Species: TROVE2 antibody was raised against Human
• Antigen Type: Recombinant protein
• Immunogen: TROVE2 antibody was raised against recombinant protein
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-TROVE2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Western Blot (WB)

(TROVE2 monoclonal antibody (M01), clone 2B4. Western blot of TROVE2 expression in HeLa.)

ELISA (EIA)

(Detection limit for recombinant GST tagged TROVE2 is approximately 0.3 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 31377800
• NCBI GeneID: 6738
• NCBI Accession #: NP_004591.2
• NCBI GenBank Nucleotide #: NM_004600.5
• UniProt Accession #: P10155; Q5LJ98; Q5LJ99; Q5LJA0; Q86WL3; Q86WL4; Q92787; Q9H1W6; B2RBB9
• NCBI Official Full Name: 60 kDa SS-A/Ro ribonucleoprotein isoform 2
• NCBI Official Synonym Full Names: TROVE domain family, member 2
• NCBI Official Symbol: TROVE2
• NCBI Official Synonym Symbols: RO60; SSA2; RORNP
• NCBI Protein Information: 60 kDa SS-A/Ro ribonucleoprotein; 60 kDa ribonucleoprotein Ro; sjoegren syndrome type A antigen; gastric cancer multi-drug resistance protein; Sjogren syndrome antigen A2 (60kD, ribonucleoprotein autoantigen SS-A/Ro)
• UniProt Protein Name: 60 kDa SS-A/Ro ribonucleoprotein
• Protein Family: 60 kDa SS-A/Ro ribonucleoprotein
• UniProt Gene Name: TROVE2
• UniProt Synonym Gene Names: RO60; SSA2; 60 kDa Ro protein; 60 kDa ribonucleoprotein Ro; RoRNP; SS-A
• UniProt Entry Name: RO60_HUMAN

Uniprot Description

SSA2: RNA-binding protein that binds to several small cytoplasmic RNA molecules known as Y RNAs. May stabilize these RNAs from degradation. Belongs to the Ro 60 kDa family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 1q31

Cellular Component: nucleoplasm; cytoplasm; ribonucleoprotein complex

Molecular Function: RNA binding; metal ion binding; U2 snRNA binding

Biological Process: transcription from RNA polymerase III promoter; immune system development; smoothened signaling pathway; response to UV

Disease: Heart Block, Congenital