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Immunohistochemistry (IHC) (Anti-DPF2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Rabbit anti-Human, Mouse Requiem / DPF2 Polyclonal Antibody | anti-DPF2 antibody

Rabbit Polyclonal to Human Requiem / DPF2

Gene Names
DPF2; REQ; UBID4; ubi-d4
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Immunoaffinity Purified
Synonyms
Requiem / DPF2; Polyclonal Antibody; Rabbit Polyclonal to Human Requiem / DPF2; Anti-Requiem / DPF2 Antibody (C-Terminus) IHC-plus; DPF2; BAF45D; REQ; UBID4; Zinc finger protein ubi-d4; BRG1-associated factor 45D; Ubi-d4; Protein requiem; Requiem; Human Requiem; anti-DPF2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
Human DPF2. Requiem antibody is predicted to not cross-react with other DPF protein family members.
Purity/Purification
Immunoaffinity Purified
Form/Format
PBS, 0.02% sodium azide
Concentration
1 mg/ml (varies by lot)
Sequence Length
391
Applicable Applications for anti-DPF2 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes
IHC-P (5 ug/ml), WB (1 - 2 ug/ml)
Usage: Tested in IHC-P with human FFPE tissues and in Western blot with mouse kidney lysates.
Target Species
Human
Immunogen Description
An 18 amino acid peptide near the carboxy terminus of human Requiem.
Immunogen Type
Synthetic peptide
Antigen Modification
C-Terminus
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-DPF2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC) (Anti-DPF2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of REQUIEM in human kidney tissue with REQUIEM antibody at 20 ug/ml.)

Immunofluorescence (IF) (Immunofluorescence of REQUIEM in human kidney tissue with REQUIEM antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot analysis of Requiem in mouse kidney tissue lysate with Requiem antibody at 1 ug/ml.)

Western Blot (WB) (Western blot analysis of Requiem in mouse kidney tissue lysate with Requiem antibody at 1 ug/ml.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
23,599 Da
NCBI Official Full Name
zinc finger protein ubi-d4
NCBI Official Synonym Full Names
D4, zinc and double PHD fingers family 2
NCBI Official Symbol
DPF2
NCBI Official Synonym Symbols
REQ; UBID4; ubi-d4
NCBI Protein Information
zinc finger protein ubi-d4; BAF45D; protein requiem; BRG1-associated factor 45D; apoptosis response zinc finger protein; requiem, apoptosis response zinc finger
UniProt Protein Name
Zinc finger protein ubi-d4
Protein Family
UniProt Gene Name
DPF2
UniProt Synonym Gene Names
BAF45D; REQ; UBID4; BAF45D
UniProt Entry Name
REQU_HUMAN

NCBI Description

The protein encoded by this gene is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It likely serves a regulatory role in rapid hematopoietic cell growth and turnover. This gene is considered a candidate gene for multiple endocrine neoplasia type I, an inherited cancer syndrome involving multiple parathyroid, enteropancreatic, and pituitary tumors. [provided by RefSeq, Jul 2008]

Uniprot Description

requiem: May be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells. Belongs to the requiem/DPF family.

Protein type: C2H2-type zinc finger protein; Apoptosis

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; centrosome; intracellular membrane-bound organelle; nuclear chromatin; cytoplasm

Molecular Function: zinc ion binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; apoptosis

Research Articles on DPF2

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Product Notes

The DPF2 dpf2 (Catalog #AAA243893) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human Requiem / DPF2 reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Requiem / DPF2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB). IHC-P (5 ug/ml), WB (1 - 2 ug/ml) Usage: Tested in IHC-P with human FFPE tissues and in Western blot with mouse kidney lysates. Researchers should empirically determine the suitability of the DPF2 dpf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Requiem / DPF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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