Sheep anti-Human F10 / Factor X Polyclonal Antibody | anti-F10 antibody

Sheep Polyclonal (IgG) to Human F10 / Factor X

Cat. Num. AAA243848

• Gene Names: F10; FX; FXA
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Immunodiffusion
• Purity: Immunoaffinity Purified
• Synonyms: F10 / Factor X; Polyclonal Antibody; Sheep Polyclonal (IgG) to Human F10 / Factor X; Anti-F10 / Factor X Antibody IHC-plus; F10; Factor X; FX; Factor X deficiency; Stuart factor; Stuart-Prower factor; Coagulation factor X; Factor Xa; FXA; Prothrombinase; Human F10; anti-F10 antibody

• Host: Sheep
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Affinity purified IgG was analyzed by immunoelectrophoresis. Only one arc was seen with normal plasma. No arc was seen with Factor X deficient plasma.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 10 mM HEPES, pH 7.4, 0.15 M sodium chloride, 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 488

• Applicable Applications for anti-F10 antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA), Immunodiffusion, Immunoelectrophoresis
• Application Notes: ELISA, ID, IE, IHC-P (5 ug/ml); Usage: Suitable for use in ELISA, Immunoelectrophoresis, Immunohistochemistry with FFPE human samples and Immunodiffusion (vs. citrated plasma).
• Target Species: Human
• Immunogen Description: Human Factor X purified from plasma, activated and active site blocked.
• Immunogen Type: Purified protein
• Immunogen: F10 / Factor X antibody was raised against human Factor X purified from plasma, activated and active site blocked.
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-F10 / Factor X antibody IHC of human liver, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-F10 / Factor X antibody IHC of human small intestine, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Product Categories/Family for anti-F10 antibody

Family: Protease; Subfamily: Serine S1

NCBI and Uniprot Product Information

• NCBI GI #: 4503625
• NCBI GeneID: 2159
• NCBI Accession #: NP_000495.1
• NCBI GenBank Nucleotide #: NM_000504.3
• UniProt Accession #: P00742; Q14340
• Molecular Weight: 54,732 Da
• NCBI Official Full Name: coagulation factor X preproprotein
• NCBI Official Synonym Full Names: coagulation factor X
• NCBI Official Symbol: F10
• NCBI Official Synonym Symbols: FX; FXA
• NCBI Protein Information: coagulation factor X; factor Xa; prothrombinase; Stuart-Prower factor
• UniProt Protein Name: Coagulation factor X
• Protein Family: Coagulation factor
• UniProt Gene Name: F10
• UniProt Entry Name: FA10_HUMAN

NCBI Description

This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. [provided by RefSeq, Jul 2008]

Uniprot Description

F10: Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. Defects in F10 are the cause of factor X deficiency (FA10D). A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis. Belongs to the peptidase S1 family.

Protein type: Secreted; EC 3.4.21.6; Protease; Lipid-binding; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: endoplasmic reticulum lumen; Golgi lumen; plasma membrane; extracellular region

Molecular Function: protein binding; serine-type endopeptidase activity; phospholipid binding; calcium ion binding

Biological Process: positive regulation of protein kinase B signaling cascade; blood coagulation, extrinsic pathway; cellular protein metabolic process; proteolysis; blood coagulation; post-translational protein modification; peptidyl-glutamic acid carboxylation; blood coagulation, intrinsic pathway; positive regulation of cell migration

Disease: Factor X Deficiency