Sheep anti-Human ACHE / Acetylcholinesterase Polyclonal Antibody | anti-ACHE antibody

Sheep Polyclonal (IgG) to Human ACHE / Acetylcholinesterase

Cat. Num. AAA243265

• Gene Names: ACHE; YT; ACEE; ARACHE; N-ACHE
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Protein G Purified
• Synonyms: ACHE / Acetylcholinesterase; Polyclonal Antibody; Sheep Polyclonal (IgG) to Human ACHE / Acetylcholinesterase; Anti-ACHE / Acetylcholinesterase Antibody IHC-plus; ACHE; ACEE; Acetylcholinesterase; N-ACHE; Yt blood group; ARACHE; YT; Human ACHE; anti-ACHE antibody

• Host: Sheep
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Acetylcholinesterase.
• Purity/Purification: Protein G Purified
• Form/Format: PBS, 0.09% sodium azide
• Concentration: 5 mg/ml (varies by lot)
• Sequence Length: 617

• Applicable Applications for anti-ACHE antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml); Usage: Suitable for use in ELISA.; ; Optimal dilution should be determined by the researcher.
• Target Species: Human
• Antigen Type: Native protein
• Immunogen: ACHE / Acetylcholinesterase antibody was raised against native protein.
• Immunogen Species: ACHE / Acetylcholinesterase antibody was produced against Human.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.

Related Product Information for anti-ACHE antibody

Acetylcholinesterase is involved in termination of signal transmission at neuromuscular junctions. Differences in assembly and localization of the multiple synaptic forms of acetylcholinesterase are thought to arise through posttranscriptional events. The Yt erythrocyte blood group antigen system resides on the acetylcholinesterase molecule.

NCBI and Uniprot Product Information

• NCBI GI #: 7710112
• NCBI GeneID: 43
• NCBI Accession #: NP_056646.1
• NCBI GenBank Nucleotide #: NM_015831.2
• UniProt Accession #: P22303; Q16169; Q29S23; Q2M324; Q504V3; Q53F46; Q86TM9; Q86YX9; Q9BXP7; A4D2E2; B7ZKZ0; D6W5X7
• NCBI Official Full Name: acetylcholinesterase isoform E4-E5
• NCBI Official Synonym Full Names: acetylcholinesterase (Yt blood group)
• NCBI Official Symbol: ACHE
• NCBI Official Synonym Symbols: YT; ACEE; ARACHE; N-ACHE
• NCBI Protein Information: acetylcholinesterase; Yt blood group; apoptosis-related acetylcholinesterase
• UniProt Protein Name: Acetylcholinesterase
• UniProt Gene Name: ACHE
• UniProt Synonym Gene Names: AChE
• UniProt Entry Name: ACES_HUMAN

NCBI Description

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq, Jul 2008]

Uniprot Description

ACHE: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. Belongs to the type-B carboxylesterase/lipase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Membrane protein, GPI anchor; EC 3.1.1.7; Lipid Metabolism - glycerophospholipid

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: Golgi apparatus; extracellular space; cell surface; membrane; perinuclear region of cytoplasm; plasma membrane; extracellular region; basal lamina; synapse; cell junction; neuromuscular junction; nucleus

Molecular Function: collagen binding; serine hydrolase activity; protein binding; protein homodimerization activity; cholinesterase activity; protein self-association; hydrolase activity; beta-amyloid binding; laminin binding; acetylcholinesterase activity; acetylcholine binding

Biological Process: nervous system development; negative regulation of synaptic transmission, cholinergic; muscle development; acetylcholine catabolic process in synaptic cleft; acetylcholine catabolic process; glycerophospholipid biosynthetic process; osteoblast development; cell proliferation; synaptic transmission; synaptogenesis; positive regulation of protein secretion; retina development in camera-type eye; amyloid precursor protein metabolic process; phospholipid metabolic process; neurotransmitter receptor biosynthetic process; receptor internalization; response to wounding; phosphatidylcholine biosynthetic process; regulation of receptor recycling; DNA replication; cell adhesion; protein tetramerization; neurotransmitter biosynthetic process

Disease: Yt Blood Group Antigen