Rabbit P2Y10 / P2RY10 Polyclonal Antibody | anti-P2Y10 antibody

Rabbit Polyclonal to Human P2Y10 / P2RY10

Cat. Num. AAA243258

• Gene Names: P2RY10; P2Y10; LYPSR2
• Reactivity: Human, Monkey
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: P2Y10 / P2RY10; Polyclonal Antibody; Rabbit Polyclonal to Human P2Y10 / P2RY10; Anti-P2Y10 / P2RY10 Antibody (Extracellular Domain) IHC-plus; P2RY10; p2RY10; p2Y-like receptor; Putative P2Y purinoceptor 10; p2Y purinoceptor 10; p2Y10; Human P2Y10; anti-P2Y10 antibody

• Host: Rabbit
• Reactivity: Human, Monkey
• Clonality: Polyclonal
• Specificity: Human P2RY10 / P2Y10. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 339

• Applicable Applications for anti-P2Y10 antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (3 ug/ml)
• Target Species: Human
• Antigen Type: Synthetic peptide
• Immunogen: P2Y10 / P2RY10 antibody was raised against synthetic 16 amino acid peptide from 2nd extracellular domain of human P2RY10 / P2Y10. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey (100%); Gibbon (94%).
• Antigen Modification: Extracellular Domain
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-P2Y10 / P2RY10 antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-P2Y10 / P2RY10 antibody IHC of human Lung, Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-P2RY10 / P2Y10 antibody IHC of human spleen, lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-P2Y10 antibody

P2Y10 is a putative Purinergic Receptor.

Product Categories/Family for anti-P2Y10 antibody

Family: GPCR; Subfamily: Purinergic

NCBI and Uniprot Product Information

• NCBI GI #: 10092633
• NCBI GeneID: 27334
• NCBI Accession #: NP_055314.1
• NCBI GenBank Nucleotide #: NM_014499.2
• UniProt Accession #: O00398; Q4VBN7; Q86V16; D3DTE5
• NCBI Official Full Name: putative P2Y purinoceptor 10
• NCBI Official Synonym Full Names: purinergic receptor P2Y, G-protein coupled, 10
• NCBI Official Symbol: P2RY10
• NCBI Official Synonym Symbols: P2Y10; LYPSR2
• NCBI Protein Information: putative P2Y purinoceptor 10; P2Y-like receptor; G-protein coupled purinergic receptor P2Y10
• UniProt Protein Name: Putative P2Y purinoceptor 10
• UniProt Gene Name: P2RY10
• UniProt Synonym Gene Names: P2Y10
• UniProt Entry Name: P2Y10_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of G-protein coupled receptors, that are preferentially activated by adenosine and uridine nucleotides. Two alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

P2RY10: Putative receptor for purines coupled to G-proteins. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1

Chromosomal Location of Human Ortholog: Xq21.1

Cellular Component: integral to membrane; plasma membrane

Molecular Function: purinergic nucleotide receptor activity, G-protein coupled