Immunohistochemistry (IHC)
(Anti-STAT5 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 40 ug/ml.)
Rabbit STAT5 A+B Polyclonal Antibody | anti-STAT5 A+B antibody
Rabbit Polyclonal to Mouse STAT5 A+B
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Immunoaffinity Purified
Synonyms
STAT5 A+B; Polyclonal Antibody; Rabbit Polyclonal to Mouse STAT5 A+B; Anti-STAT5 A+B Antibody (phospho-Tyr694) IHC-plus; Mouse STAT5 A+B; anti-STAT5 A+B antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
A BLAST analysis was used to suggest cross-reactivity with Stat5 alpha from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Stat5 alpha from other sources has not been determined.
Purity/Purification
Immunoaffinity Purified
Form/Format
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-STAT5 A+B antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA (1:25000), IHC-P (20 - 40 ug/ml), WB (1:500 - 1:2000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
Target Species
Mouse
Immunogen Description
Synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5 alpha protein.
Immunogen Type
Synthetic peptide
Immunogen
STAT5 A+B antibody was raised against synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5 alpha protein.
Antigen Modification
pTyr694
Preparation and Storage
+4 degree C or -20 degree C, Avoid repeated freezing and thawing.
Immunohistochemistry (IHC)
(Anti-STAT5 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 40 ug/ml.)
Immunohistochemistry (IHC)
(Anti-STAT5 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 40 ug/ml.)
Western Blot (WB)
(Anti-STAT5 pY694 Antibody - Western Blot. Western blot of affinity purified anti-Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kD) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1000 dilution, overnight at 4C. Personal Communication from Ana Gamero, CCR-NCI, Bethesda, MD.)
Western Blot (WB)
(Anti-STAT5 pY694 Antibody - Western Blot. Western blot of affinity purified anti-Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kD) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1000 dilution, overnight at 4C. Personal Communication from Ana Gamero, CCR-NCI, Bethesda, MD.)
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Product Notes
The STAT5 A+B (Catalog #AAA242042) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Mouse STAT5 A+B reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's STAT5 A+B can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). ELISA (1:25000), IHC-P (20 - 40 ug/ml), WB (1:500 - 1:2000) Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the STAT5 A+B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STAT5 A+B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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