Goat ROBO2 Polyclonal Antibody | anti-ROBO2 antibody

Goat Polyclonal to Human ROBO2

Cat. Num. AAA241970

• Gene Names: ROBO2; SAX3
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Gorilla, Monkey, Bat, Bovine, Pig, Chicken (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: ROBO2; Polyclonal Antibody; Goat Polyclonal to Human ROBO2; Anti-ROBO2 Antibody (Internal) IHC-plus; KIAA1568; Roundabout homolog 2; SAX3; Human ROBO2; anti-ROBO2 antibody

• Host: Goat
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Gorilla, Monkey, Bat, Bovine, Pig, Chicken (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human ROBO2.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 1394

• Applicable Applications for anti-ROBO2 antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA (1:16000), IHC-P (5 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-NQSQRPRPTKKHK from an internal region (near the C-terminus) of human ROBO2 (NP_002933.1). Percent identity by BLAST analysis: Mouse, Rat, Opossum (100%).
• Immunogen Type: Synthetic peptide
• Immunogen: ROBO2 antibody was raised against synthetic peptide C-NQSQRPRPTKKHK from an internal region (near the C-terminus) of human ROBO2 (NP_002933.1). Percent identity by BLAST analysis: Mouse, Rat, Opossum (100%); Human, Gorilla, Monkey, Marmoset, Elephant, Panda, Bovine, Bat, Pig, Chicken (92%); Dog, Rabbit (85%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-ROBO2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 193083163
• NCBI GeneID: 6092
• NCBI Accession #: NP_001122401.1
• NCBI GenBank Nucleotide #: NM_001128929.3
• UniProt Accession #: Q9HCK4; O43608; Q19AB4; Q19AB5
• Molecular Weight: 153,120 Da
• NCBI Official Full Name: roundabout homolog 2 isoform ROBO2a
• NCBI Official Synonym Full Names: roundabout, axon guidance receptor, homolog 2 (Drosophila)
• NCBI Official Symbol: ROBO2
• NCBI Official Synonym Symbols: SAX3
• NCBI Protein Information: roundabout homolog 2
• UniProt Protein Name: Roundabout homolog 2
• Protein Family: Roundabout
• UniProt Gene Name: ROBO2
• UniProt Synonym Gene Names: KIAA1568
• UniProt Entry Name: ROBO2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the ROBO family, part of the immunoglobulin superfamily of proteins that are highly conserved from fly to human. The encoded protein is a transmembrane receptor for the slit homolog 2 protein and functions in axon guidance and cell migration. Mutations in this gene are associated with vesicoureteral reflux, characterized by the backward flow of urine from the bladder into the ureters or the kidney. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

ROBO2: Receptor for SLIT2, and probably SLIT1, which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. Defects in ROBO2 are the cause of vesicoureteral reflux type 2 (VUR2). VUR is a complex, genetically heterogeneous developmental disorder characterized by the retrograde flow of urine from the bladder into the ureter and is associated with reflux nephropathy, the cause of 15% of end-stage renal disease in children and young adults. A chromosomal aberration involving ROBO2 is a cause of multiple congenital abnormalities, including severe bilateral VUR with ureterovesical junction defects. Translocation t(Y;3)(p11;p12) with PCDH11Y. This translocation disrupts ROBO2 and produces dominant-negative ROBO2 proteins that abrogate SLIT- ROBO signaling in vitro. Belongs to the immunoglobulin superfamily. ROBO family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis; Cell development/differentiation

Chromosomal Location of Human Ortholog: 3p12.3

Cellular Component: cell surface; integral to membrane; axolemma

Molecular Function: identical protein binding; protein binding; axon guidance receptor activity

Biological Process: axon guidance; central nervous system development; olfactory bulb interneuron development; negative regulation of synaptogenesis; axon midline choice point recognition; cellular response to hormone stimulus; negative regulation of negative chemotaxis; ureteric bud development; positive regulation of axonogenesis; brain development; homophilic cell adhesion; metanephros development; retinal ganglion cell axon guidance

Disease: Vesicoureteral Reflux 2