Rabbit CCR8 Polyclonal Antibody | anti-CCR8 antibody

Rabbit Polyclonal to Human CCR8

Cat. Num. AAA241419

• Gene Names: CCR8; CY6; TER1; CCR-8; CKRL1; CDw198; CMKBR8; GPRCY6; CMKBRL2; CC-CKR-8
• Reactivity: Human; Predicted: Monkey
• Applications: Immunohistochemistry, Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: CCR8; Polyclonal Antibody; Rabbit Polyclonal to Human CCR8; Anti-CCR8 Antibody (Cytoplasmic Domain) IHC-plus; C-C chemokine receptor type 8; CC chemokine receptor CHEMR1; CC-CKR-8; Chemokine (C-C) receptor 8; CDw198 antigen; CKRL1; CMKBR8; Chemokine receptor-like 1; CMKBRL2; CKR-L1; GPRCY6; TER1; C-C CKR-8; CC chemokine receptor 8; Human CCR8; anti-CCR8 antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Monkey
• Clonality: Polyclonal
• Specificity: Human CCR8. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 355

• Applicable Applications for anti-CCR8 antibody: Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-Paraffin), ELISA (EIA)
• Application Notes: IHC; IHC-Paraffin (5 ug/ml); ELISA; (optimal dilution to be determined by the researcher); ; Immunohistochemistry: MBS241419 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS241419 was determined to be 5 ug/ml.
• Immunogen Species: CCR8 antibody was raised against Human
• Antigen Type: Synthetic peptide
• Immunogen: CCR8 antibody was raised against synthetic 19 amino acid peptide from 3rd cytoplasmic domain of human CCR8. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey (100%); Gibbon, Marmoset (95%); Bovine, Horse (84%).
• Epitope: Cytoplasmic Domain
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Product Categories/Family for anti-CCR8 antibody

Family: GPCR; Subfamily: Chemokine

NCBI and Uniprot Product Information

• NCBI GI #: 4885121
• NCBI GeneID: 1237
• NCBI Accession #: NP_005192.1
• NCBI GenBank Nucleotide #: NM_005201.3
• UniProt Accession #: P51685; Q3KNQ8; Q3KNR3; Q9BYX5; B2RC64
• NCBI Official Full Name: C-C chemokine receptor type 8
• NCBI Official Synonym Full Names: chemokine (C-C motif) receptor 8
• NCBI Official Symbol: CCR8
• NCBI Official Synonym Symbols: CY6; TER1; CCR-8; CKRL1; CDw198; CMKBR8; GPRCY6; CMKBRL2; CC-CKR-8
• NCBI Protein Information: C-C chemokine receptor type 8; CC chemokine receptor 8; chemokine receptor-like 1; chemokine (C-C) receptor 8; CC chemokine receptor CHEMR1; CC-chemokine receptor chemr1; chemokine (C-C) receptor-like 2
• UniProt Protein Name: C-C chemokine receptor type 8
• Protein Family: C-C chemokine receptor
• UniProt Gene Name: CCR8
• UniProt Synonym Gene Names: CKRL1; CMKBR8; CMKBRL2; C-C CKR-8; CC-CKR-8; CCR-8; CKR-L1; GPRCY6
• UniProt Entry Name: CCR8_HUMAN

NCBI Description

This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors are important for the migration of various cell types into the inflammatory sites. This receptor protein preferentially expresses in the thymus. I-309, thymus activation-regulated cytokine (TARC) and macrophage inflammatory protein-1 beta (MIP-1 beta) have been identified as ligands of this receptor. Studies of this receptor and its ligands suggested its role in regulation of monocyte chemotaxis and thymic cell apoptosis. More specifically, this receptor may contribute to the proper positioning of activated T cells within the antigenic challenge sites and specialized areas of lymphoid tissues. This gene is located at the chemokine receptor gene cluster region. [provided by RefSeq, Jul 2008]

Uniprot Description

CCR8: Receptor for the chemokine CCL1/SCYA1/I-309. May regulate monocyte chemotaxis and thymic cell line apoptosis. Alternative coreceptor with CD4 for HIV-1 infection. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Receptor, GPCR; Motility/polarity/chemotaxis; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 3p22

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: C-C chemokine receptor activity; chemokine receptor activity; coreceptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; immune response; chemotaxis; cell adhesion