Rabbit anti-Human, Mouse RREB1 Polyclonal Antibody | anti-RREB1 antibody

Rabbit Polyclonal to Mouse RREB1

Cat. Num. AAA240857

• Gene Names: RREB1; HNT; FINB; LZ321; Zep-1; RREB-1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: RREB1; Polyclonal Antibody; Rabbit Polyclonal to Mouse RREB1; Anti-RREB1 Antibody IHC-plus; DNA-binding protein; FINB; Hindsight homolog; Zep-1; HNT; LZ321; RREB-1; Mouse RREB1; anti-RREB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: Mouse RREB1 protein.
• Purity/Purification: Protein A Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1742

• Applicable Applications for anti-RREB1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:2000 - 1:10000), IHC-P (10 ug/ml), WB (1:500 - 1:2000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Mouse
• Immunogen Description: Recombinant mouse RREB1.
• Immunogen Type: Recombinant protein
• Immunogen: RREB1 antibody was raised against recombinant mouse RREB1.
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-RREB1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Anti-RREB1 Antibody - Western Blot. Western blot of Protein A Purified anti-RREB1 antibody shows detection of a predominant band believed to be RREB1 in various cell lysates (1 - HEK293, 2 - RFP-RREB transfected HEK293, 3 - M460 and 4 - T1165). All lysates were loaded at 20 ug per lane and separated by SDS-PAGE. After transfer to nitrocellulose, the membrane was probed with the primary antibody diluted to 1:1000. The membrane was washed and reacted with IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers as indicated. Personal Communication, Shuling Zhang, CCR, NCI, Bethesda, MD.)

Related Product Information for anti-RREB1 antibody

RREB1is a zinc finger transcription factor that binds to RAS-responsive elements (RREs) of gene promoters. It has been shown that the calcitonin gene promoter contains an RRE and that the encoded protein binds there and increases expression of calcitonin, which may be involved in Ras/Raf-mediated cell differentiation. Multiple transcript variants encoding several different isoforms have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 51173737
• NCBI GeneID: 6239
• NCBI Accession #: NP_001003699.1
• NCBI GenBank Nucleotide #: NM_001003699.3
• UniProt Accession #: Q92766; O75567; O75568; Q5VYB2; Q6BEP5; Q6BEP6; Q6BEP8; Q86SU2; Q9Y474; A2RRF5; E2GM80; E2GM81
• Molecular Weight: 34,614 Da
• NCBI Official Full Name: ras-responsive element-binding protein 1 isoform 1
• NCBI Official Synonym Full Names: ras responsive element binding protein 1
• NCBI Official Symbol: RREB1
• NCBI Official Synonym Symbols: HNT; FINB; LZ321; Zep-1; RREB-1
• NCBI Protein Information: ras-responsive element-binding protein 1; hindsight homolog; DNA-binding protein; finger protein in nuclear bodies; raf-responsive zinc finger protein LZ321; zinc finger motif enhancer binding protein 1; zinc finger motif enhancer-binding protein 1; zinc finger motif-enhancer binding-protein 1
• UniProt Protein Name: Ras-responsive element-binding protein 1
• Protein Family: Ras-responsive element-binding protein
• UniProt Gene Name: RREB1
• UniProt Synonym Gene Names: FINB; RREB-1; Zep-1
• UniProt Entry Name: RREB1_HUMAN

NCBI Description

The protein encoded by this gene is a zinc finger transcription factor that binds to RAS-responsive elements (RREs) of gene promoters. It has been shown that the calcitonin gene promoter contains an RRE and that the encoded protein binds there and increases expression of calcitonin, which may be involved in Ras/Raf-mediated cell differentiation. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]

Uniprot Description

RREB1: Transcription factor that binds specifically to the RAS- responsive elements (RRE) of gene promoters. May be involved in Ras/Raf-mediated cell differentiation by enhancing calcitonin expression. Represses the angiotensinogen gene. Negatively regulates the transcriptional activity of AR. Potentiates the transcriptional activity of NEUROD1. Belongs to the krueppel C2H2-type zinc-finger protein family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; C2H2-type zinc finger protein; Motility/polarity/chemotaxis; DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 6p25

Cellular Component: nucleoplasm; nuclear body; cytoplasm; nucleolus; nuclear speck; nucleus

Molecular Function: metal ion binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; multicellular organismal development; Ras protein signal transduction; positive regulation of mammary gland epithelial cell proliferation; negative regulation of transcription from RNA polymerase II promoter