Rabbit FANCF Polyclonal Antibody | anti-FANCF antibody

Rabbit Polyclonal to Human FANCF

Cat. Num. AAA240747

• Gene Names: FANCF; FAF
• Reactivity: Chimpanzee, Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: FANCF; Polyclonal Antibody; Rabbit Polyclonal to Human FANCF; Anti-FANCF Antibody IHC-plus; Fanconi anemia group F protein; Protein FACF; FAF; Human FANCF; anti-FANCF antibody

• Host: Rabbit
• Reactivity: Chimpanzee, Human
• Clonality: Polyclonal
• Specificity: An internal amino acid sequence of human FANCF.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 374

• Applicable Applications for anti-FANCF antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:15000 - 1:60000), IF, IHC-P (2.5 ug/ml), WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human FANCF.
• Immunogen Type: Synthetic peptide
• Immunogen: FANCF antibody was raised against synthetic peptide from human FANCF.
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-FANCF antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Anti-FANCF Antibody - Western Blot. Western blot of affinity purified anti-FANCF antibody shows detection of FANCF present in a lysate prepared from a Fanconi anemia complementation group F patient lymphoblast after retroviral correction using hFANCF cDNA (lanes 3 and 4). This band (indicated by arrowhead) is approximately 42.3 kD in size. The band is not detected in FA-F a lymphoblast lysate that is not corrected for the deletion and does not express the FANCF protein (lanes 1 and 2). Lanes 2 and 4 represent lysates taken from lymphoblasts after 40 J/m2 UV irradiation, whereas lanes 1 and 3 received no irradiation. No apparent difference was noted upon irradiation. The strong band at ~60kD appears to be non-specific. Personal communication, N. Howlett, University of Rhode Island, Kingston, RI.)

NCBI and Uniprot Product Information

• NCBI GI #: 12232377
• NCBI GeneID: 2188
• NCBI Accession #: NP_073562.1
• NCBI GenBank Nucleotide #: NM_022725.3
• UniProt Accession #: Q9NPI8; Q52LM0
• Molecular Weight: 42,254 Da
• NCBI Official Full Name: Fanconi anemia group F protein
• NCBI Official Synonym Full Names: Fanconi anemia, complementation group F
• NCBI Official Symbol: FANCF
• NCBI Official Synonym Symbols: FAF
• NCBI Protein Information: Fanconi anemia group F protein
• UniProt Protein Name: Fanconi anemia group F protein
• Protein Family: Fanconi anemia group F protein
• UniProt Gene Name: FANCF
• UniProt Synonym Gene Names: Protein FACF
• UniProt Entry Name: FANCF_HUMAN

NCBI Description

The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group F. [provided by RefSeq, Jul 2008]

Uniprot Description

FANCF: DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Defects in FANCF are the cause of Fanconi anemia complementation group F (FANCF). A disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: nucleoplasm

Molecular Function: protein binding; ubiquitin-protein ligase activity

Biological Process: ovarian follicle development; protein ubiquitination; spermatogenesis; DNA repair

Disease: Fanconi Anemia, Complementation Group F; Tracheoesophageal Fistula With Or Without Esophageal Atresia