Goat anti-Human AHSG / Fetuin Polyclonal Antibody | anti-AHSG antibody

Goat Polyclonal to Human AHSG / Fetuin

Cat. Num. AAA240601

• Gene Names: AHSG; AHS; A2HS; HSGA; FETUA
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Immunoprecipitation, ELISA
• Purity: Purified IgG
• Synonyms: AHSG / Fetuin; Polyclonal Antibody; Goat Polyclonal to Human AHSG / Fetuin; Anti-AHSG / Fetuin Antibody IHC-plus; A2HS; Alpha-2-Z-globulin; Fetuin; Fetuin-A; AHS; Alpha-2-HS-glycoprotein; Ba-alpha-2-glycoprotein; FETUA; HSGA; Human AHSG; anti-AHSG antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recombinant human fetuin (a2-HS glycoprotein) processed to remove a 40 amino acid residue bridging peptide resulting in the mature form of the protein.
• Purity/Purification: Purified IgG
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)

• Applicable Applications for anti-AHSG antibody: Immunohistochemistry - Paraffin (IHC-P), Western Blot (WB), Immmunoprecipitation (IP), ELISA (EIA); ; Optimal dilution to be determined by the researcher.
• Application Notes: IHC-Paraffin: 2.5 ug/ml; WB: 1:10000-1:20000; ; Summary: Immunohistochemistry: MBS240601 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS240601 was determined to be 2.5 ug/ml.
• Host Note: AHSG / Fetuin A antibody was produced in Goat
• Immunogen Species: AHSG / Fetuin A antibody was raised against Human
• Antigen Type: Recombinant protein
• Immunogen: AHSG / Fetuin A antibody was raised against recombinant human AHSG / Fetuin A
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Related Product Information for anti-AHSG antibody

Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue.

NCBI and Uniprot Product Information

• NCBI GI #: 156523970
• NCBI GeneID: 197
• NCBI Accession #: NP_001613.2
• NCBI GenBank Nucleotide #: NM_001622.2
• UniProt Accession #: P02765; O14961; O14962; Q9P152; A8K9N6; B2R7G1; AAB29984
• NCBI Official Full Name: alpha-2-HS-glycoprotein preproprotein
• NCBI Official Synonym Full Names: alpha-2-HS-glycoprotein
• NCBI Official Symbol: AHSG
• NCBI Official Synonym Symbols: AHS; A2HS; HSGA; FETUA
• NCBI Protein Information: alpha-2-HS-glycoprotein; fetuin-A; alpha-2-Z-globulin; ba-alpha-2-glycoprotein
• UniProt Protein Name: Alpha-2-HS-glycoprotein
• Protein Family: Alpha-2-HS-glycoprotein
• UniProt Gene Name: AHSG
• UniProt Synonym Gene Names: FETUA
• UniProt Entry Name: FETUA_HUMAN

NCBI Description

Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. However, its exact significance is still obscure. [provided by RefSeq, Jul 2008]

Uniprot Description

FETUA: Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions. Alpha-2-HS glycoprotein derives from this precursor, when the connecting peptide is cleaved off. The two chains A and B are held together by a single disulfide bond. Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins. Belongs to the fetuin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: extracellular space; extracellular region

Molecular Function: kinase inhibitor activity; cysteine protease inhibitor activity

Biological Process: pinocytosis; negative regulation of bone mineralization; ossification; positive regulation of phagocytosis; regulation of inflammatory response; acute-phase response; negative regulation of insulin receptor signaling pathway; negative regulation of phosphorylation; regulation of bone mineralization; skeletal development